Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.108 extracted from

  • Schlegel-Haueter, S.; Hore, P.; Kerry, K.R.; Semenza, G.
    The preparation of lactase and glucoamylase of rat small intestine (1972), Biochim. Biophys. Acta, 258, 506-519.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21
-
lactose
-
Homo sapiens
21
-
lactose
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Homo sapiens
-
small intestine
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, stable for several months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Homo sapiens D-glucose + D-galactose
-
?
lactose + H2O
-
Rattus norvegicus D-glucose + D-galactose
-
?
phlorizin + H2O
-
Homo sapiens phloretin + D-glucose
-
?
phlorizin + H2O
-
Rattus norvegicus phloretin + D-glucose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
about 30% loss of lactase activity after 30 min Rattus norvegicus
46
-
35 min, about 50% loss of phlorizin hydrolase activity. About 70% loss of lactase activity after 25 min Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
30% loss of activity after 0.5 min, 55% loss of activity after 120 min Rattus norvegicus