Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.108 extracted from

  • Ramaswamy, S.; Radhakrishnan, A.N.
    Lactase-phlorizin hydrolase complex from monkey small intestine. Purification, properties and evidence for two catalytic sites (1975), Biochim. Biophys. Acta, 403, 446-455.
    View publication on PubMed
Search for more literature for 3.2.1.108

Inhibitors

Inhibitors Comment Organism Structure
cerebroside bovine brain cerebroside Platyrrhini
phlorizin competitive inhibitor of lactose hydrolysis Platyrrhini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 phlorizin
-
 Platyrrhini
2.78
-
 cellobiose
-
 Platyrrhini
21.8
-
 lactose
-
 Platyrrhini

Organism

Organism UniProt Comment Textmining
Platyrrhini
-
 lactase-phlorizin hydrolase complex
-

Purification (Commentary)

Purification (Comment) Organism
-
 Platyrrhini

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
 Platyrrhini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.1
-
 lactase Platyrrhini
450
-
 phlorizin hydrolase Platyrrhini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O 16% of the activity with lactose Platyrrhini 2 beta-D-glucose
-
 ?
lactose + H2O
-
 Platyrrhini D-glucose + D-galactose most active substrate ?
methyl-beta-D-glucoside + H2O 7.3% of the activity with lactose Platyrrhini methanol + beta-D-glucose
-
 ?
o-nitrophenyl-beta-galactoside + H2O 15.4% of the activity with lactose Platyrrhini o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-glucoside + H2O 13% of the activity with lactose Platyrrhini o-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-galactoside + H2O 9.3% of the activity with lactose Platyrrhini p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-glucoside + H2O 12.7% of the activity with lactose Platyrrhini p-nitrophenol + beta-D-glucose
-
 ?
phlorizin + H2O at 2.3% of the activity with lactose Platyrrhini phloretin + D-glucose
-
 ?
salicin + H2O 1.6% of the activity with lactose Platyrrhini ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 lactase and phlorizin hydrolase activity are identically heat inactivated Platyrrhini