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Literature summary for 3.2.1.106 extracted from
- Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63 (2008), J. Mol. Biol., 381, 116-128.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli B834 (DE3) for selenomethionine derivates | Escherichia coli |
| expression in Escherichia coli BL21 (DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 230 | - |
nigerose | - |
Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kojibiose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| Maltoheptaose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| Maltohexaose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| maltopentaose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| maltose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| Maltotetraose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| maltotriose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| nigerose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
| trehalose | pH 5.0, 35°C, 30 min | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YgjK | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
pH 5.0 | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
loss of activity at 50°C, pH 7.5, 30 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
42 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 10 | residual activity higher than 90%, 4°C, 30 min | Escherichia coli |
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Release 2023.1 (January 2023)
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