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Literature summary for 3.2.1.106 extracted from

  • Bause, E.; Schweden, J.; Gross, A.; Orthen, B.
    Purification and characterization of trimming glucosidase I from pig liver (1989), Eur. J. Biochem., 183, 661-669.
    View publication on PubMed
Search for more literature for 3.2.1.106

General Stability

General Stability Organism
native form of glucosidase I is unusually resistant to non-specific proteolysis, partial digestion with trypsin requires rather high concentrations of the protease, at high, that is equimolar, concentrations of trypsin enzyme is cleaved into a defined and enzymatically active mixture of protein fragments with molecular mass of 69 kDa, 45 kDa and 29 kDa Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin strong inhibition Bos taurus
1-deoxynojirimycin strong inhibition Homo sapiens
1-deoxynojirimycin strong inhibition; strong inhibition, Ki = 0.0021 mM Sus scrofa
Cu2+
-
 Bos taurus
Cu2+ partial inhibition Sus scrofa
kojibiose alpha-1,2-linked glucose disaccharide Bos taurus
kojibiose alpha-1,2-linked glucose disaccharide; Ki = 0.095 mM Sus scrofa
additional information
-
 Bos taurus
additional information
-
 Homo sapiens
additional information no inhibition by 1 mM EDTA or 1 mM o-phenanthroline Sus scrofa
N,N-dimethyl-1-deoxynojirimycin strong inhibition Bos taurus
N,N-dimethyl-1-deoxynojirimycin strong inhibition Homo sapiens
N,N-dimethyl-1-deoxynojirimycin strong inhibition; strong inhibition, Ki = 0.0005 mM Sus scrofa
N-(5-carboxypentyl)-1-deoxynojirimycin strong inhibition Bos taurus
N-(5-carboxypentyl)-1-deoxynojirimycin strong inhibition; strong specific inhibition, Ki = 0.00045 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Sus scrofa
additional information
-
 additional information kinetic data Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Homo sapiens 5783
-
endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Sus scrofa 5783
-
endoplasmic reticulum rough endoplasmic reticulum Sus scrofa 5783
-
endoplasmic reticulum rough endoplasmic reticulum Bos taurus 5783
-
membrane membrane-bound Homo sapiens 16020
-
membrane membrane-bound Sus scrofa 16020
-
membrane membrane-bound Bos taurus 16020
-
microsome
-
 Homo sapiens
-
-
microsome
-
 Sus scrofa
-
-
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions not required Homo sapiens
additional information metal ions not required Sus scrofa
additional information metal ions not required Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Homo sapiens hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Bos taurus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Homo sapiens specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Bos taurus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Homo sapiens involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Homo sapiens involved in processing of asparagine-linked oligosaccharides ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
Glc3Man9GlcNAc2 + H2O Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Homo sapiens involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Homo sapiens involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Sus scrofa glucose processing is essential for pro-receptor transport and maturation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein with high-mannose oligosaccharides Homo sapiens
glycoprotein with high-mannose oligosaccharides Sus scrofa
glycoprotein with high-mannose oligosaccharides Bos taurus
glycoprotein N-glycoprotein, one N-linked high-mannose sugar chain of less than 3 kDA Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
intestine calf Bos taurus
-
kidney calf Bos taurus
-
liver
-
 Homo sapiens
-
liver
-
 Sus scrofa
-
liver calf Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, stable for several weeks Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Homo sapiens D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Sus scrofa D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Bos taurus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
additional information Glc1Man9GlcNAc2: not a substrate Homo sapiens ?
-
 ?
additional information Glc1Man9GlcNAc2: not a substrate Sus scrofa ?
-
 ?
additional information Glc1Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Homo sapiens ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Sus scrofa ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Bos taurus ?
-
 ?
additional information methylumbelliferyl-beta-glucosides: no substrates Sus scrofa ?
-
 ?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Homo sapiens ?
-
 ?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Sus scrofa ?
-
 ?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Homo sapiens ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Sus scrofa ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
additional information p-nitrophenyl-beta-D-glucopyranoside: not a substrate Sus scrofa ?
-
 ?
additional information p-nitrophenyl-beta-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
additional information substrate specificities Sus scrofa ?
-
 ?
additional information substrate specificities Bos taurus ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Homo sapiens ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Homo sapiens ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
additional information glucose processing is essential for pro-receptor transport and maturation Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
?
-
 Bos taurus
? x * 85000, SDS-PAGE, reducing conditions Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.6 7.3 half-maximal activity at, steep decrease in activity on acidic side Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00045
-
 N-(5-carboxypentyl)-1-deoxynojirimycin strong specific inhibition Sus scrofa
0.0005
-
 N,N-dimethyl-1-deoxynojirimycin strong inhibition Sus scrofa