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Literature summary for 3.2.1.106 extracted from

  • Rush, J.S.; Waechter, C.J.
    Topological studies on the enzymes catalyzing the biosynthesis of Glc-P-dolichol and the triglucosyl cap of Glc3Man9GlcNAc2-P-P-dolichol in microsomal vesicles from pig brain: use of the processing glucosidases I/II as latency markers (1998), Glycobiology, 8, 1207-1213.
    View publication on PubMed
Search for more literature for 3.2.1.106

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation, maximal activity in the presence of 5-10 mg/ml Triton X-100 Rattus norvegicus
Triton X-100 activation, maximal activity in the presence of 0.5-2 mg/ml Triton X-100 Sus scrofa

Application

Application Comment Organism
analysis using the processing glucosidase activity as latency marker for topological studies with microsomal vesicles from nongluconeogenic tissues in addition to brain and cultured mammalian cells lacking glucose 6-phosphate phosphatase, to assess the intactness of microsomal vesicles Rattus norvegicus
analysis using the processing glucosidase activity as latency marker for topological studies with microsomal vesicles from nongluconeogenic tissues in addition to brain and cultured mammalian cells lacking glucose 6-phosphate phosphatase, to assess the intactness of microsomal vesicles Sus scrofa

General Stability

General Stability Organism
resistant to trypsin or subtilisin in sealed microsomal vesicles, in unsealed vesicles by the addition of 2 mg/ml Triton X-100, inactivation within 5 min Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin
-
 Rattus norvegicus
1-deoxynojirimycin 0.01 mM: 50% inhibition Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum endoplasmic reticulum vesicles Rattus norvegicus 5783
-
endoplasmic reticulum endoplasmic reticulum vesicles Sus scrofa 5783
-
endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Rattus norvegicus 5783
-
endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Sus scrofa 5783
-
membrane membrane-bound Rattus norvegicus 16020
-
membrane membrane-bound Sus scrofa 16020
-
microsome microsomal vesicles Rattus norvegicus
-
-
microsome microsomal vesicles Sus scrofa
-
-
additional information topological studies Rattus norvegicus
-
-
additional information topological studies Sus scrofa
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Rattus norvegicus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-
kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O
-
 Rattus norvegicus D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O
-
 Sus scrofa D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
 1-deoxynojirimycin
-
 Sus scrofa