Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | activation, required in order to measure glucosidase activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | 5 mM: 25% inhibition | Rattus norvegicus | |
D-mannose | 5 mM: 25% inhibition | Rattus norvegicus | |
additional information | no inhibition by EDTA | Rattus norvegicus | |
p-nitrophenyl-alpha-D-glucoside | 5 mM | Rattus norvegicus | |
p-nitrophenyl-beta-D-glucoside | 5 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | rough and smooth endoplasmic reticulum | Rattus norvegicus | 5783 | - |
membrane | integral membrane protein | Rattus norvegicus | 16020 | - |
membrane | microsomal membranes | Rattus norvegicus | 16020 | - |
microsome | localized in rough and smooth microsomes, located on the cisternal surface of microsomal vesicles | Rattus norvegicus | - |
- |
additional information | subcellular distribution and orientation | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Rattus norvegicus | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Rattus norvegicus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
additional information | Rattus norvegicus | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | ? | - |
? | |
additional information | Rattus norvegicus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose | glucosidase I is a specific alpha-(1,2)-glucosidase | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Rattus norvegicus | D-glucose + Glc2Man9GlcNAc | - |
? | |
Glc3Man9GlcNAc2 + H2O | - |
Rattus norvegicus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | Rattus norvegicus | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | involved in processing of asparagine-linked oligosaccharides | Rattus norvegicus | ? | - |
? | |
Glc3Man9GlcNAc2-lipid + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Rattus norvegicus | D-glucose + Glc2Man9GlcNAc2-lipid | - |
? | |
Glc3Man9GlcNAc2-peptide + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Rattus norvegicus | D-glucose + Glc2Man9GlcNAc2-peptide | - |
? | |
additional information | catalyzes initial step in post-glycosylation processing of cellular glycoprotein | Rattus norvegicus | ? | - |
? | |
additional information | involved in processing of asparagine-linked oligosaccharides | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | broad pH optimum between | Rattus norvegicus |