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Literature summary for 3.2.1.105 extracted from

  • Hemscheid, T.; Zenk, M.H.
    Glucosidases involved in indole alkaloid biosynthesis of Catharanthus cell culture (1980), FEBS Lett., 110, 187-191.
    View publication on PubMed
Search for more literature for 3.2.1.105

Inhibitors

Inhibitors Comment Organism Structure
D(+)-gluconic acid delta-lactone enzyme I is much less sensitive than enzyme II Catharanthus roseus
strictosidine enzyme I shows substrate inhibition at 1 mM, enzyme II not Catharanthus roseus
tryptamine 2.5 mM, 15% inhibition of enzyme I and II Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 strictosidine enzyme II Catharanthus roseus
0.2
-
 strictosidine enzyme I Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
 enzyme I, gel filtration Catharanthus roseus
450000
-
 above, enzyme II, gel filtration Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
strictosidine Catharanthus roseus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Amsonia orientalis
-
 weak activity
-
Amsonia salicifolia
-
-
-
Catharanthus ovalis
-
-
-
Catharanthus pusillus
-
-
-
Catharanthus roseus
-
-
-
Catharanthus trichophyllus
-
-
-
Rauvolfia vomitoria
-
 weak activity
-
Vinca major
-
 weak activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
strictosidine
-
 Catharanthus roseus ?
-
 ?
vincoside + H2O hydrolyzed at less than 10% of strictosidine Catharanthus roseus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.4 enzyme I and II Catharanthus roseus