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Literature summary for 3.2.1.104 extracted from

  • Watanabe, T.; Tani, M.; Ishibashi, Y.; Endo, I.; Okino, N.; Ito, M.
    Ergosteryl-beta-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae (2015), Glycobiology, 25, 1079-1089 .
    View publication on PubMed
Search for more literature for 3.2.1.104

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h) Saccharomyces cerevisiae 5829
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membrane the intracellular localization is changed depending on the growth phase. Distributed in the cytosol until the initial logarithmic phase (12 h), then delivered to an organelle with a granule structure in the middle logarithmic phase (14 h), and finally localized at the vacuole membranes in the stationary phase (18-20 h) Saccharomyces cerevisiae 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
138000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40566
-
-
Saccharomyces cerevisiae ATCC 204508 P40566
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae D-glucose + 4-methylumbelliferone
-
 ?
4-methylumbelliferyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae ATCC 204508 D-glucose + 4-methylumbelliferone
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae D-glucose + 4-nitrophenol
-
 ?
4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae ATCC 204508 D-glucose + 4-nitrophenol
-
 ?
cholesteryl 3-O-beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae D-glucose + cholesterol
-
 ?
cholesteryl 3-O-beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae ATCC 204508 D-glucose + cholesterol
-
 ?
ergosteryl 3-O-beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae D-glucose + ergosterol
-
 ?
ergosteryl 3-O-beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae ATCC 204508 D-glucose + ergosterol
-
 ?
glucosylceramide + H2O
-
 Saccharomyces cerevisiae D-glucose + ceramide
-
 ?
glucosylceramide + H2O
-
 Saccharomyces cerevisiae ATCC 204508 D-glucose + ceramide
-
 ?
sitosteryl 3-O-beta-D-glucoside + H2O
-
 Saccharomyces cerevisiae D-glucose + sitosterol
-
 ?

Subunits

Subunits Comment Organism
? x * 138000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Egh1
-
 Saccharomyces cerevisiae
ergosteryl-beta-glucosidase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the disruption of EGH1 in Saccharomyces cerevisiae BY4741 (egh1DELTA) results in the accumulation of ergosteryl 3-O-beta-glucoside and fragmentation of vacuoles Saccharomyces cerevisiae
physiological function involved in sterylglucoside catabolism and vacuole formation Saccharomyces cerevisiae