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Literature summary for 3.2.1.104 extracted from
- Purification and some properties of steryl beta-D-glucoside hydrolase from Sinapis alba seedlings (1978), Phytochemistry, 17, 1533-1537.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 45% stimulation at 0.1 mM, hydrolysis of cholesteryl beta-D-glucoside. No effect on hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba |  |
| ethanol | 64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5alpha-cholestanol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
| campesterol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
| cholesterol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
| ethanol | 39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba | |
| gluconolactone | 50% inhibition at 4 mM, hydrolysis of cholesteryl beta-D-glucoside. 50% inhibition at 2 mM, hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba | |
| sitosterol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | - |
Sinapis alba | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Sinapis alba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinapis alba | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Sinapis alba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Sinapis alba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3621 | - |
- |
Sinapis alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl beta-D-glucoside + H2O | - |
Sinapis alba | D-glucose + cholesterol | - |
? | |
| p-nitrophenyl beta-D-glucoside + H2O | - |
Sinapis alba | D-glucose + p-nitrophenol | - |
? | |
| sitosteryl beta-D-glucoside + H2O | - |
Sinapis alba | D-glucose + sitosterol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
hydrolysis of cholesteryl beta-D-glucoside | Sinapis alba |
| 60 | - |
hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 5.6 | hydrolysis of cholesteryl beta-D-glucoside or p-nitrophenyl beta-D-glucoside | Sinapis alba |
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Release 2023.1 (January 2023)
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