BRENDA - Enzyme Database show
show all sequences of 3.2.1.10

Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae

Deng, X.; Petitjean, M.; Teste, M.; Kooli, W.; Tranier, S.; Francois, J.; Parrou, J.; FEBS Open Bio 4, 200-212 (2014)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
L240P
similar to wild-type
Saccharomyces cerevisiae
R279Q
displays substrate specificities and specific activities close to those of isoform Ima2
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-nitrophenyl alpha-D-glucopyranoside
-
Saccharomyces cerevisiae
isomaltose
-
Saccharomyces cerevisiae
maltose
competitive; competitive; mixed
Saccharomyces cerevisiae
methyl alpha-D-glucopyranoside
-
Saccharomyces cerevisiae
palatinose
-
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.35
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
0.48
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
0.58
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
0.89
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
7
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
11
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
12
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
13
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
17
-
isomaltose
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
18
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
27
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
28
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
37
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
72
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
83
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
116
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
128
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
144
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
147
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
191
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
P0CW40
isoform Ima3
-
Saccharomyces cerevisiae
P40884
isoform Ima5
-
Saccharomyces cerevisiae
P53051
isoform Ima1
-
Saccharomyces cerevisiae
Q08295
isoform Ima2
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
3.9
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
5
-
substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
5.8
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
7.2
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
7.6
-
substrate isomaltriose, pH 7.0, 30°C
Saccharomyces cerevisiae
7.7
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
7.9
-
substrate isomaltriose, pH 7.0, 30°C
Saccharomyces cerevisiae
8.6
-
substrate isomaltriose, pH 7.0, 30°C
Saccharomyces cerevisiae
10
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
10.2
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
11
-
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
18.6
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
19.7
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
39
-
substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
52
-
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C; substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
54
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
55
-
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
60
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
65
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
74
-
substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
729747
Saccharomyces cerevisiae
4-nitrophenol + alpha-D-glucopyranose
-
-
-
?
isomaltose + H2O
-
729747
Saccharomyces cerevisiae
2 D-glucose
-
-
-
?
isomaltriose + H2O
-
729747
Saccharomyces cerevisiae
?
-
-
-
?
methyl alpha-D-glucopyranoside + H2O
-
729747
Saccharomyces cerevisiae
methanol + alpha-D-glucopyranose
-
-
-
?
additional information
isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis-Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose
729747
Saccharomyces cerevisiae
?
-
-
-
-
additional information
isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis–Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose
729747
Saccharomyces cerevisiae
?
-
-
-
-
palatinose + H2O
-
729747
Saccharomyces cerevisiae
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
-
729747
Saccharomyces cerevisiae
D-glucose + D-fructose
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
36
-
-
Saccharomyces cerevisiae
43
-
-
Saccharomyces cerevisiae
46
-
-
Saccharomyces cerevisiae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
29.9
-
melting temperature, pH 8
Saccharomyces cerevisiae
33.2
-
melting temperature, pH 8
Saccharomyces cerevisiae
35.7
-
melting temperature, pH 8
Saccharomyces cerevisiae
36.3
-
melting temperature, pH 7
Saccharomyces cerevisiae
36.6
-
melting temperature, pH 6
Saccharomyces cerevisiae
37
-
half-life 23.2 min; half-life 26.1 min; half-life 27.4 min; half-life 2.9 min
Saccharomyces cerevisiae
38.8
-
melting temperature, pH 7
Saccharomyces cerevisiae
40
-
half-life 0.8 min; half-life 17.6 min; half-life 18.5 min; half-life 7.6 min
Saccharomyces cerevisiae
41.6
-
melting temperature, pH 8
Saccharomyces cerevisiae
46.2
-
melting temperature, pH 7
Saccharomyces cerevisiae
46.6
-
melting temperature, pH 7
Saccharomyces cerevisiae
47
-
melting temperature, pH 6
Saccharomyces cerevisiae
55.1
-
melting temperature, pH 6
Saccharomyces cerevisiae
55.5
-
melting temperature, pH 6
Saccharomyces cerevisiae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
6
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
7.1
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
8.7
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
14
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
14
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
18
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
21
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
25
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
51
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
55
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
56
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
78
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
84
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
88
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
97
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
101
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
103
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
104
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
7.6
-
-
Saccharomyces cerevisiae
8
-
-
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2
5
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
9.8
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
12
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
188
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
246
-
isomaltose
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
248
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
574
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
600
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
686
-
palatinose
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
690
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L240P
similar to wild-type
Saccharomyces cerevisiae
R279Q
displays substrate specificities and specific activities close to those of isoform Ima2
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-nitrophenyl alpha-D-glucopyranoside
-
Saccharomyces cerevisiae
isomaltose
-
Saccharomyces cerevisiae
maltose
competitive
Saccharomyces cerevisiae
maltose
mixed
Saccharomyces cerevisiae
methyl alpha-D-glucopyranoside
-
Saccharomyces cerevisiae
palatinose
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2
5
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
9.8
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
12
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
188
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
246
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
248
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
574
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
600
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
686
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
690
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.35
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
0.48
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
0.58
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
0.89
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
7
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
11
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
12
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
13
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
17
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
18
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
27
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
28
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
37
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
72
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
83
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
116
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
128
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
144
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
147
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
191
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
3.9
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
5
-
substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
5.8
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
7.2
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
7.6
-
substrate isomaltriose, pH 7.0, 30°C
Saccharomyces cerevisiae
7.7
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
7.9
-
substrate isomaltriose, pH 7.0, 30°C
Saccharomyces cerevisiae
8.6
-
substrate isomaltriose, pH 7.0, 30°C
Saccharomyces cerevisiae
10
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
10.2
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
11
-
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
18.6
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
19.7
-
substrate sucrose, pH 7.0, 30°C
Saccharomyces cerevisiae
39
-
substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
52
-
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C; substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
54
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
55
-
substrate methyl alpha-D-glucopyranoside, pH 7.0, 30°C
Saccharomyces cerevisiae
60
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
65
-
substrate isomaltose, pH 7.0, 30°C
Saccharomyces cerevisiae
74
-
substrate palatinose, pH 7.0, 30°C
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
729747
Saccharomyces cerevisiae
4-nitrophenol + alpha-D-glucopyranose
-
-
-
?
isomaltose + H2O
-
729747
Saccharomyces cerevisiae
2 D-glucose
-
-
-
?
isomaltriose + H2O
-
729747
Saccharomyces cerevisiae
?
-
-
-
?
methyl alpha-D-glucopyranoside + H2O
-
729747
Saccharomyces cerevisiae
methanol + alpha-D-glucopyranose
-
-
-
?
additional information
isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis-Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose
729747
Saccharomyces cerevisiae
?
-
-
-
-
additional information
isoforms Ima1, Ima2, Ima3 and Ima5 exhibit a preference for the alpha-(1,6) disaccharides isomaltose and palatinose, with Michaelis–Menten kinetics and inhibition at high substrates concentration. They are also able to hydrolyze trisaccharides bearing an alpha-(1,6) linkage, but also alpha-(1,2), alpha-(1,3) and alpha-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. No substrate: melibiose
729747
Saccharomyces cerevisiae
?
-
-
-
-
palatinose + H2O
-
729747
Saccharomyces cerevisiae
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
-
729747
Saccharomyces cerevisiae
D-glucose + D-fructose
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
36
-
-
Saccharomyces cerevisiae
43
-
-
Saccharomyces cerevisiae
46
-
-
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
29.9
-
melting temperature, pH 8
Saccharomyces cerevisiae
33.2
-
melting temperature, pH 8
Saccharomyces cerevisiae
35.7
-
melting temperature, pH 8
Saccharomyces cerevisiae
36.3
-
melting temperature, pH 7
Saccharomyces cerevisiae
36.6
-
melting temperature, pH 6
Saccharomyces cerevisiae
37
-
half-life 26.1 min
Saccharomyces cerevisiae
37
-
half-life 27.4 min
Saccharomyces cerevisiae
37
-
half-life 23.2 min
Saccharomyces cerevisiae
37
-
half-life 2.9 min
Saccharomyces cerevisiae
38.8
-
melting temperature, pH 7
Saccharomyces cerevisiae
40
-
half-life 17.6 min
Saccharomyces cerevisiae
40
-
half-life 18.5 min
Saccharomyces cerevisiae
40
-
half-life 7.6 min
Saccharomyces cerevisiae
40
-
half-life 0.8 min
Saccharomyces cerevisiae
41.6
-
melting temperature, pH 8
Saccharomyces cerevisiae
46.2
-
melting temperature, pH 7
Saccharomyces cerevisiae
46.6
-
melting temperature, pH 7
Saccharomyces cerevisiae
47
-
melting temperature, pH 6
Saccharomyces cerevisiae
55.1
-
melting temperature, pH 6
Saccharomyces cerevisiae
55.5
-
melting temperature, pH 6
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
6
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
7.1
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
8.7
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
14
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
14
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
18
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
21
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
25
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
51
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
55
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
56
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
78
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
84
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
88
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
97
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
101
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
103
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
104
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Saccharomyces cerevisiae
7.6
-
-
Saccharomyces cerevisiae
8
-
-
Saccharomyces cerevisiae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.02
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.16
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.21
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.22
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.22
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.35
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.38
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.41
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.8
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.9
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
3.1
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C; pH 7.0, 30°C
Saccharomyces cerevisiae
4.3
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
5
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
5.6
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
5.7
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
8
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
9.4
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
16
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
20
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
28
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.02
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.16
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.21
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.22
-
isomaltriose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.22
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.35
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.38
-
sucrose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.41
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.8
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
0.9
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
3.1
-
methyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
4.3
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
5
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
5.6
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
5.7
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
8
-
isomaltose
pH 7.0, 30°C
Saccharomyces cerevisiae
9.4
-
palatinose
pH 7.0, 30°C
Saccharomyces cerevisiae
15
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
16
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
20
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
28
-
4-nitrophenyl alpha-D-glucopyranoside
pH 7.0, 30°C
Saccharomyces cerevisiae
Other publictions for EC 3.2.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750760
Wang
A new GH13 alpha-glucosidase ...
Bacillus pseudofirmus, Bacillus pseudofirmus 703
Int. J. Biol. Macromol.
101
973-982
2017
-
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1
-
1
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1
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2
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4
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1
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8
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1
1
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1
1
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1
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1
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1
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2
-
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1
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-
-
-
8
-
1
1
-
-
1
1
-
-
-
2
2
-
-
-
750849
Delgado
Genetic and biochemical chara ...
Lactobacillus plantarum subsp. plantarum, Lactobacillus plantarum subsp. plantarum ATCC 149917, Lactobacillus plantarum subsp. plantarum LL441
Int. J. Food Microbiol.
246
32-39
2017
-
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1
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2
1
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3
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1
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1
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12
2
1
1
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1
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1
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2
1
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1
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1
-
12
2
1
1
-
-
1
-
-
-
-
1
1
-
-
-
751091
Gericke
Structure-function analysis o ...
Homo sapiens
J. Biol. Chem.
292
11070-11078
2017
-
-
1
-
11
-
1
3
2
-
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1
-
1
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-
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1
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2
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1
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1
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1
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11
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1
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3
2
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1
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1
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2
-
1
-
-
-
1
-
-
-
-
5
5
-
-
-
729378
Natori
Synthesis and biological evalu ...
Rattus norvegicus
Bioorg. Med. Chem. Lett.
24
3298-3301
2014
-
-
-
-
-
-
3
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-
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1
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1
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3
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3
3
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1
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-
-
-
-
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-
-
-
-
-
-
-
-
729747
Deng
Similarities and differences i ...
Saccharomyces cerevisiae
FEBS Open Bio
4
200-212
2014
-
-
-
-
2
-
5
21
-
-
-
-
-
4
-
-
-
-
-
-
21
-
27
-
3
-
14
21
3
-
-
-
10
-
-
-
-
-
-
-
2
-
-
17
14
22
-
-
-
-
-
-
-
-
-
-
21
-
27
-
3
-
20
22
3
-
-
-
-
-
-
-
21
22
725283
Choi
Identification and characteriz ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639
J. Bacteriol.
195
1789-1799
2013
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1
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1
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3
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2
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1
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2
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1
4
1
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3
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1
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1
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1
-
3
-
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1
-
2
-
1
4
1
-
-
3
-
-
-
-
1
1
-
-
1
-
-
729812
Rodriguez
Functional analysis of sucrase ...
Homo sapiens
Hum. Mol. Genet.
22
2273-2282
2013
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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1
-
-
-
4
-
-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730248
Diaz-Sotomayor
Maltase-glucoamylase modulates ...
Mus musculus
J. Pediatr. Gastroenterol. Nutr.
57
704-712
2013
-
1
-
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
714471
Jones
Mapping the intestinal alpha-g ...
Homo sapiens
Bioorg. Med. Chem.
19
3929-3934
2011
-
-
1
-
-
-
7
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
7
6
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717357
Eskandari
Selectivity of 3-O-methylponko ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
6491-6494
2011
-
1
-
-
-
-
10
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
1
-
-
-
-
-
6
10
4
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
704667
Sim
Structural basis for substrate ...
Homo sapiens
J. Biol. Chem.
285
17763-17770
2010
-
-
1
1
-
-
2
3
-
-
1
-
-
1
-
-
1
-
-
1
-
-
4
-
-
-
-
3
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
2
2
3
-
-
1
-
-
-
-
1
-
1
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
717588
Yamamoto
Crystal structures of isomalta ...
Saccharomyces cerevisiae
FEBS J.
277
4205-4214
2010
-
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1
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1
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-
-
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3
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-
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1
1
1
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-
-
1
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-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
717798
Teste
Characterization of a new mult ...
Saccharomyces cerevisiae
J. Biol. Chem.
285
26815-26824
2010
-
-
1
-
-
-
-
-
-
-
-
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-
1
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-
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1
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1
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1
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1
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-
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-
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-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
690556
Pokusaeva
Characterization of two novel ...
Bifidobacterium breve, Bifidobacterium breve UCC2003
Appl. Environ. Microbiol.
75
1135-1143
2009
-
-
1
-
-
-
-
12
-
-
3
-
-
4
-
-
1
-
-
-
16
-
28
-
2
-
-
12
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
12
-
-
4
-
-
-
-
2
-
-
16
-
28
-
2
-
-
12
2
-
-
-
-
-
-
-
-
-
691394
Yorita
Induction of histone acetylati ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biosci. Biotechnol. Biochem.
73
933-935
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
162
-
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702506
Wetzel
Impact of glycosylation and de ...
Homo sapiens
Biol. Chem.
390
545-549
2009
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
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1
-
-
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677995
Suzuki
Histone H3 modifications and C ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
369
788-793
2008
-
-
-
-
-
-
-
-
-
-
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1
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-
1
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-
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-
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
690249
Yamamoto
Crystallization and preliminar ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. F
64
1024-1026
2008
-
-
1
1
-
-
-
-
-
-
-
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-
2
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1
-
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-
-
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1
-
1
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1
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-
-
-
-
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-
-
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-
-
-
-
-
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-
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-
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691212
Minami
Effect of five-membered sugar ...
Rattus sp.
Bioorg. Med. Chem.
16
2734-2740
2008
-
-
-
-
-
-
11
-
-
-
-
-
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1
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-
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-
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-
-
-
-
-
-
-
-
-
-
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-
10
-
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-
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10
11
-
-
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-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693790
Quezada-Calvillo
Luminal starch substrate 'brak ...
Homo sapiens
J. Nutr.
138
685-692
2008
-
-
-
-
-
-
-
5
1
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2
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1
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6
-
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1
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1
5
1
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1
-
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6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677473
Bosse
Gata4 and Hnf1alpha are partia ...
Mus musculus
Am. J. Physiol. Gastrointest. Liver Physiol.
292
G1302-G1314
2007
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-
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-
-
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1
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1
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1
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1
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1
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677949
Gu
HNF-1alpha participates in glu ...
Homo sapiens
Biochem. Biophys. Res. Commun.
353
617-622
2007
1
1
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2
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3
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1
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681827
Naumoff
-
Structure and evolution of the ...
Bos taurus, Canis lupus familiaris, Homo sapiens, Macaca mulatta, Mus musculus, Oryctolagus cuniculus, Pan troglodytes, Rattus norvegicus, Suncus murinus
Mol. Biol.
41
962-973
2007
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9
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Sucrase-isomaltase gene expres ...
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van den Broek
Cloning and characterization o ...
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2003
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2003
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A glutamine to proline exchang ...
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655073
Watanabe
Oligo-1,6-glucosidase from a t ...
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2002
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655196
Oslancova
Oligo-1,6-glucosidase and neop ...
Bacillus cereus, Bacillus coagulans, Bacillus flavocaldarius, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) F5, Bacillus subtilis, Erwinia rhapontici, Parageobacillus thermoglucosidasius
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Substrate recognition by three ...
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Takesue
Comparison of sucrase-free iso ...
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2001
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Watanabe
Identification of catalytic an ...
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Inhibitory effects of ellagi- ...
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2001
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Sucrase-isomaltase is an adeno ...
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2001
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Watanabe
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Wiltz
Expression of enzymatically ac ...
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Galand
First purification and charact ...
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Beaulieu
Detection and characterization ...
Homo sapiens
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Yamamoto
Purification and properties of ...
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Broyart
Molecular cloning and characte ...
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Watanabe
Cloning and expression in Esch ...
Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
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Plant
A cell-associated oligo-1,6-al ...
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Lecommandeur
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Purification of germinated bar ...
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326244
Suzuki
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A strong correlation between t ...
Bacillus cereus, Bacillus coagulans, Bacillus flavocaldarius, Bacillus thermoamyloliquefaciens KP1071, Parageobacillus thermoglucosidasius
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Kakefuda
Chloroplast and extrachloropla ...
Pisum sativum
Planta
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Suzuki
Purification and characterizat ...
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11
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35
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11
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326247
Yoshigi
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326248
Suzuki
-
The lack of identity between B ...
Geobacillus stearothermophilus, Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
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1984
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3
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326249
Wacker
A two-active site one-polypept ...
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J. Biol. Chem.
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1984
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8
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326250
Suzuki
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A simplified method of the pur ...
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Eur. J. Appl. Microbiol. Biotechnol.
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1983
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326251
Suzuki
-
Assignment of a p-nitrophenyl- ...
Bacillus cereus
Biochim. Biophys. Acta
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476-483
1982
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6
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16
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7
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6
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326252
Suzuki
Abnormally high tolerance agai ...
Bacillus cereus, Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
Biochim. Biophys. Acta
705
124-126
1982
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Sjöström
A fully active, two-active-sit ...
Sus scrofa
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1980
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136506
Hauri
Biogenesis of intestinal plasm ...
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Proc. Natl. Acad. Sci. USA
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1979
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326253
Skovbjerg
Does sucrase-isomaltase always ...
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326254
Suzuki
Hydrolysis of low molecular we ...
Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
Biochim. Biophys. Acta
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62-66
1979
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Marshall
Solubilization of porcine inte ...
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326257
Quaroni
Affinity labeling of the activ ...
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326258
Asp
Separation of human small-inte ...
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Khan
Purification and characterizat ...
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