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Literature summary for 3.2.1.10 extracted from

  • Sim, L.; Willemsma, C.; Mohan, S.; Naim, H.Y.; Pinto, B.M.; Rose, D.R.
    Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains (2010), J. Biol. Chem., 285, 17763-17770.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Drosophila melanogaster S2 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sucrase-isomaltase in apo form and in complex with kotalanol, hanging drop vapor diffusion method, using 0.1 M MgCl2, 0.1 M bis-Tris propane, pH 7.0, 15% (w/v) polyethylene glycol 4000 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
 Homo sapiens
kotalanol
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 4-nitrophenyl alpha-D-glucopyranoside in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
7.1
-
 maltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
11.1
-
 isomaltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14410
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-Sepharose resin column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-D-glucopyranose
-
 ?
isomaltose + H2O
-
 Homo sapiens 2 D-glucose
-
 ?
maltose + H2O
-
 Homo sapiens 2 D-glucose
-
 ?
additional information N-terminal sucrase-isomaltase has a broad specificity for both alpha-1,4- and alpha-1,6-oligosaccharides Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
sucrase-isomaltase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
 4-nitrophenyl alpha-D-glucopyranoside in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
97
-
 isomaltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
137
-
 maltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
 kotalanol
-
 Homo sapiens
0.014
-
 acarbose
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 isomaltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
13
-
 4-nitrophenyl alpha-D-glucopyranoside in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens
19
-
 maltose in 100 mM MES buffer, pH 6.5, at 37°C Homo sapiens