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Literature summary for 3.2.1.10 extracted from
- Cloning and characterization of two alpha-glucosidases from Bifidobacterium adolescentis DSM20083 (2003), Appl. Microbiol. Biotechnol., 61, 55-60.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of genetic library, DNA sequence determination and analysis of gene aglA, amino acid sequence determination, expression of the truncated enzyme fused to the N-terminus of lacZ in Escherichia coli XL1-Blue | Bifidobacterium adolescentis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.05 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.5, 30°C | Bifidobacterium adolescentis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Bifidobacterium adolescentis |
| 71000 | - |
1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation | Bifidobacterium adolescentis |
| 72138 | - |
1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation | Bifidobacterium adolescentis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium adolescentis | Q9AF93 | strain DSM 20083, gene aglA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Bifidobacterium adolescentis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl alpha-D-glucoside + H2O | - |
Bifidobacterium adolescentis | 4-methylumbelliferol + alpha-D-glucose | - |
? | |
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Bifidobacterium adolescentis | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| isomaltose + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
| isomaltriose + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
| additional information | substrate specificity, enzyme is also capable to perform a transglycosylation producing oligosaccharides from trehalose and with low activity from sucrose, but no hydrolytic activity is detected with sucrose | Bifidobacterium adolescentis | ? | - |
? | |
| trehalose + H2O | low activity | Bifidobacterium adolescentis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation | Bifidobacterium adolescentis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AglA | - |
Bifidobacterium adolescentis |
| More | the enzyme belongs to family 13 of glycosyl gydrolases | Bifidobacterium adolescentis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bifidobacterium adolescentis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Bifidobacterium adolescentis |
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