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Literature summary for 3.2.1.10 extracted from
- Assignment of a p-nitrophenyl-alpha-D-glucopyranoside hydrolyzing alpha-glucosidase of Bacillus cereus ATCC 7064 to an exo-oligo-1,6-glucosidase (1982), Biochim. Biophys. Acta, 704, 476-483.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Bacillus cereus |  |
| Co2+ | 96% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| Cu2+ | 95% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| D-glucose | - |
Bacillus cereus | |
| EDTA | no inhibition by 2 mM at pH 7.5 | Bacillus cereus | |
| Fe2+ | complete inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| glucono-delta-lactone | - |
Bacillus cereus | |
| Hg2+ | complete inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| Mn2+ | 40% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| p-chloromercuribenzoate | no inhibition by 2 mM with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| p-nitrophenol | - |
Bacillus cereus | |
| Pb2+ | 90% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
| phenyl alpha-D-glucoside | - |
Bacillus cereus | |
| Tris | 93% inhibition by 5 mM | Bacillus cereus | |
| Urea | 50% inactivation after treatment with 1.42 M at 25°C or 0.74 M at 35°C for 5 h | Bacillus cereus | |
| Zn2+ | 96% inhibition by 2 mM in 20 mM borate buffer, pH 7.5 with p-nitrophenyl-alpha-D-glucopyranoside as substrate | Bacillus cereus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Bacillus cereus | |
| 2.2 | - |
phenyl-alpha-D-glucopyranoside | - |
Bacillus cereus | |
| 2.8 | - |
isomaltotetraose | - |
Bacillus cereus | |
| 3 | - |
isomaltotriose | - |
Bacillus cereus | |
| 4.6 | - |
isomaltopentaose | - |
Bacillus cereus | |
| 6.2 | - |
isomaltose | - |
Bacillus cereus | |
| 11 | - |
isomaltohexaose | - |
Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | 62000 | SDS-PAGE | Bacillus cereus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | fairly stable for 5 h at 4°C in 20% ethanol , 50% inactivation after 5 h treatment with 17% ethanol at 25°C and pH 6.8 | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
ATCC 7064 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, 6 months, no loss of activity | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-limit dextrins + H2O | - |
Bacillus cereus | D-glucose | - |
? | |
| isomaltohexaose + H2O | - |
Bacillus cereus | 6 alpha-D-glucose | - |
? | |
| isomaltopentaose + H2O | - |
Bacillus cereus | 5 alpha-D-glucose | - |
? | |
| isomaltosaccharides + H2O | n = 2-6 | Bacillus cereus | D-glucose | - |
? | |
| isomaltose + H2O | - |
Bacillus cereus | 2 D-glucose | - |
? | |
| isomaltotetraose + H2O | - |
Bacillus cereus | 4 alpha-D-glucose | - |
? | |
| isomaltotriose + H2O | - |
Bacillus cereus | 3 alpha-D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | D-glucose + p-nitrophenol | - |
? | |
| panose + H2O | - |
Bacillus cereus | D-glucose + maltose | - |
? | |
| phenyl-alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | D-glucose + phenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucosidase | - |
Bacillus cereus |
| dextrin 6-alpha-D-glucanohydrolase | - |
Bacillus cereus |
| exo-oligo-1,6-glucosidase | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 53 | half-optima at 27°C and 53°C, no activity at 60°C | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 13 | 55 | heating for 10 min in 33.3 mM phosphate buffer, pH 6.8: no loss of activity from 13 to 40°C, 87% of activity at 45°C, 56% at 48°C, 25% at 50°C, no activity at 55°C | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.9 | 9.5 | half-optima at pH 5.5 and 8.2 | Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 9 | stable at 25°C for 15 h | Bacillus cereus |
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