Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.10 extracted from

  • Wacker, H.; Aggeler, R.; Kretchmer, N.; O'Neill, B.; Takesue, Y.; Semenza, G.
    A two-active site one-polypeptide enzyme: the isomaltase from sea lion small intestinal brush-border membrane. Its possible phylogenetic relationship with sucrase-isomaltase (1984), J. Biol. Chem., 259, 4878-4884.
    View publication on PubMed
Search for more literature for 3.2.1.10

Inhibitors

Inhibitors Comment Organism Structure
conduritol-B-epoxide complete inactivation by 0.024 mM Callorhinus ursinus
conduritol-B-epoxide complete inactivation by 0.024 mM Zalophus californianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 maltose 33 mM sodium maleate buffer, pH 6.8 Zalophus californianus
2.5
-
 isomaltose 33 mM sodium maleate buffer, pH 6.8 Zalophus californianus
3.5
-
 palatinose 33 mM sodium maleate buffer, pH 6.8 Zalophus californianus

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border brush-border membrane Zalophus californianus 5903
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
245000
-
 SDS-PAGE Zalophus californianus

Organism

Organism UniProt Comment Textmining
Callorhinus ursinus
-
 Alaskan fur seal
-
Zalophus californianus
-
 sea lion
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zalophus californianus
-
 Callorhinus ursinus

Reaction

Reaction Comment Organism Reaction ID
alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose enzyme from intestinal mucosa also catalyzes the reaction of sucrase alpha-glucosidase, EC 3.2.1.48 Zalophus californianus
a
alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose enzyme from intestinal mucosa also catalyzes the reaction of sucrase alpha-glucosidase, EC 3.2.1.48 Callorhinus ursinus
a

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
 Zalophus californianus
-
small intestine
-
 Callorhinus ursinus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 with isomaltose as substrate Zalophus californianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltosaccharides + H2O n = 2, isomaltose Zalophus californianus D-glucose
-
 ?
isomaltosaccharides + H2O n = 2, isomaltose Callorhinus ursinus D-glucose
-
 ?
isomaltose + H2O
-
 Zalophus californianus 2 D-glucose
-
 ?
isomaltose + H2O
-
 Callorhinus ursinus 2 D-glucose
-
 ?
maltose + H2O
-
 Zalophus californianus 2 D-glucose
-
 ?
palatinose + H2O
-
 Zalophus californianus D-glucose + fructose
-
 ?
palatinose + H2O
-
 Callorhinus ursinus D-glucose + fructose
-
 ?
sucrose + H2O small activity Callorhinus ursinus D-glucose + D-fructose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 245000, SDS-PAGE Zalophus californianus

Synonyms

Synonyms Comment Organism
isomaltase
-
 Zalophus californianus
isomaltase
-
 Callorhinus ursinus
Sea lion isomaltase
-
 Zalophus californianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6.4
-
 Zalophus californianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.3 7.8 stable at 37°C for 1 h Zalophus californianus