BRENDA - Enzyme Database show
show all sequences of 3.2.1.10

Purification and characterization of Bacillus coagulans oligo-1,6-glucosidase

Suzuki, Y.; Tomura, Y.; Eur. J. Biochem. 158, 77-83 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ba2+
72% inhibition by 2 mM BaCl2
Bacillus coagulans
Ba2+
-
Bacillus cereus
Ca2+
29% inhibition by 2 mM CaCl2
Bacillus coagulans
Cd2+
complete inhibition
Bacillus coagulans
Co2+
-
Bacillus cereus
Co2+
complete inhibition
Bacillus coagulans
Cu2+
-
Bacillus cereus
Cu2+
96% inhibition by 2 mM CuCl2
Bacillus coagulans
D-glucose
52% inhibition by 10 mM, competitive
Bacillus coagulans
D-glucose
-
Bacillus cereus
EDTA
no inhibition by 2 mM at pH 7.5
Bacillus coagulans
EDTA
no inhibition by 2 mM at pH 7.5
Bacillus cereus
Fe3+
86% inhibition by 2 mM FeCl3
Bacillus coagulans
glucono-delta-lactone
57% inhibition by 10 mM, competitive
Bacillus coagulans
glucono-delta-lactone
-
Bacillus cereus
Hg2+
-
Bacillus cereus
Hg2+
99% inhibition by 2 mM HgCl2
Bacillus coagulans
Mg2+
42% inhibition by 2 mM MgCl2
Bacillus coagulans
Mn2+
92% inhibition by 2 mM MbCl2
Bacillus coagulans
Ni2+
94% inhibition by 2 mM NiCl2
Bacillus coagulans
p-nitrophenol
complete inhibition by 2 mM
Bacillus coagulans
p-nitrophenol
-
Bacillus cereus
Pb2+
-
Bacillus cereus
Pb2+
complete inhibition
Bacillus coagulans
phenol
in combination with Tris most potent inhibitor
Bacillus coagulans
phenyl alpha-D-glucoside
58% inhibition by 10 mM
Bacillus coagulans
phenyl alpha-D-glucoside
-
Bacillus cereus
Phenyl alpha-maltoside
92% inhibition by 10 mM, noncompetitive
Bacillus coagulans
Sn2+
complete inhibition
Bacillus coagulans
Tris
-
Bacillus cereus
Tris
89% inhibition by 5 mM, competitive
Bacillus coagulans
Urea
-
Bacillus cereus
Urea
50% inactivation after treatment with 4.6 M for 5 h at 25°C and pH 6.8
Bacillus coagulans
Zn2+
-
Bacillus cereus
Zn2+
complete inhibition
Bacillus coagulans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
p-nitrophenyl alpha-D-glucopyranoside
-
Bacillus coagulans
3
-
isomaltose
-
Bacillus coagulans
4.4
-
isomaltotriose
-
Bacillus coagulans
7.4
-
isomaltotetraose
-
Bacillus coagulans
13
-
isomaltopentaose
-
Bacillus coagulans
15
-
isomaltohexaose
-
Bacillus coagulans
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Ethanol
-
Bacillus cereus
Ethanol
50% inactivation after 5 h treatment with 32% ethanol at 25°C and pH 6.8
Bacillus coagulans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
-
ATCC 7064
-
Bacillus coagulans
-
ATCC 7050
-
Purification (Commentary)
Commentary
Organism
-
Bacillus coagulans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-limit dextrins + H2O
-
326246
Bacillus cereus
D-glucose
-
326246
Bacillus cereus
?
alpha-limit dextrins + H2O
-
326246
Bacillus coagulans
D-glucose
-
326246
Bacillus coagulans
?
isomaltosaccharides + H2O
n = 2-6
326246
Bacillus cereus
D-glucose
-
326246
Bacillus cereus
?
isomaltosaccharides + H2O
n = 2-6
326246
Bacillus coagulans
D-glucose
-
326246
Bacillus coagulans
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
326246
Bacillus cereus
D-glucose + p-nitrophenol
-
326246
Bacillus cereus
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
326246
Bacillus coagulans
D-glucose + p-nitrophenol
-
326246
Bacillus coagulans
?
panose + H2O
-
326246
Bacillus cereus
D-glucose + maltose
-
326246
Bacillus cereus
?
panose + H2O
-
326246
Bacillus coagulans
D-glucose + maltose
-
326246
Bacillus coagulans
?
phenyl-alpha-D-glucopyranoside + H2O
-
326246
Bacillus coagulans
D-glucose + phenol
-
326246
Bacillus coagulans
?
sucrose + H2O
-
326246
Bacillus coagulans
D-glucose + D-fructose
-
326246
Bacillus coagulans
?
turanose + H2O
-
326246
Bacillus coagulans
D-glucose + D-fructose
-
326246
Bacillus coagulans
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
62
-
-
Bacillus coagulans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
47
75
half-optima at 47°C and 75°C, no activity at 78°C
Bacillus coagulans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
53
-
stable at pH 6.8 for 10 min
Bacillus coagulans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
-
Bacillus coagulans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.1
10.2
half-optima at pH 4.8 and 7.3
Bacillus coagulans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9.1
50% inactivation at pH 5.0 and 9.1 after incubation at 34°C for 15 h
Bacillus coagulans
6.5
7.3
50% inactivation at pH 6.5 and 7.3 after incubation at 34°C for 15 h
Bacillus cereus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ba2+
72% inhibition by 2 mM BaCl2
Bacillus coagulans
Ba2+
-
Bacillus cereus
Ca2+
29% inhibition by 2 mM CaCl2
Bacillus coagulans
Cd2+
complete inhibition
Bacillus coagulans
Co2+
-
Bacillus cereus
Co2+
complete inhibition
Bacillus coagulans
Cu2+
-
Bacillus cereus
Cu2+
96% inhibition by 2 mM CuCl2
Bacillus coagulans
D-glucose
52% inhibition by 10 mM, competitive
Bacillus coagulans
D-glucose
-
Bacillus cereus
EDTA
no inhibition by 2 mM at pH 7.5
Bacillus coagulans
EDTA
no inhibition by 2 mM at pH 7.5
Bacillus cereus
Fe3+
86% inhibition by 2 mM FeCl3
Bacillus coagulans
glucono-delta-lactone
57% inhibition by 10 mM, competitive
Bacillus coagulans
glucono-delta-lactone
-
Bacillus cereus
Hg2+
-
Bacillus cereus
Hg2+
99% inhibition by 2 mM HgCl2
Bacillus coagulans
Mg2+
42% inhibition by 2 mM MgCl2
Bacillus coagulans
Mn2+
92% inhibition by 2 mM MbCl2
Bacillus coagulans
Ni2+
94% inhibition by 2 mM NiCl2
Bacillus coagulans
p-nitrophenol
complete inhibition by 2 mM
Bacillus coagulans
p-nitrophenol
-
Bacillus cereus
Pb2+
-
Bacillus cereus
Pb2+
complete inhibition
Bacillus coagulans
phenol
in combination with Tris most potent inhibitor
Bacillus coagulans
phenyl alpha-D-glucoside
58% inhibition by 10 mM
Bacillus coagulans
phenyl alpha-D-glucoside
-
Bacillus cereus
Phenyl alpha-maltoside
92% inhibition by 10 mM, noncompetitive
Bacillus coagulans
Sn2+
complete inhibition
Bacillus coagulans
Tris
-
Bacillus cereus
Tris
89% inhibition by 5 mM, competitive
Bacillus coagulans
Urea
-
Bacillus cereus
Urea
50% inactivation after treatment with 4.6 M for 5 h at 25°C and pH 6.8
Bacillus coagulans
Zn2+
-
Bacillus cereus
Zn2+
complete inhibition
Bacillus coagulans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
p-nitrophenyl alpha-D-glucopyranoside
-
Bacillus coagulans
3
-
isomaltose
-
Bacillus coagulans
4.4
-
isomaltotriose
-
Bacillus coagulans
7.4
-
isomaltotetraose
-
Bacillus coagulans
13
-
isomaltopentaose
-
Bacillus coagulans
15
-
isomaltohexaose
-
Bacillus coagulans
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Ethanol
-
Bacillus cereus
Ethanol
50% inactivation after 5 h treatment with 32% ethanol at 25°C and pH 6.8
Bacillus coagulans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus coagulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-limit dextrins + H2O
-
326246
Bacillus cereus
D-glucose
-
326246
Bacillus cereus
?
alpha-limit dextrins + H2O
-
326246
Bacillus coagulans
D-glucose
-
326246
Bacillus coagulans
?
isomaltosaccharides + H2O
n = 2-6
326246
Bacillus cereus
D-glucose
-
326246
Bacillus cereus
?
isomaltosaccharides + H2O
n = 2-6
326246
Bacillus coagulans
D-glucose
-
326246
Bacillus coagulans
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
326246
Bacillus cereus
D-glucose + p-nitrophenol
-
326246
Bacillus cereus
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
326246
Bacillus coagulans
D-glucose + p-nitrophenol
-
326246
Bacillus coagulans
?
panose + H2O
-
326246
Bacillus cereus
D-glucose + maltose
-
326246
Bacillus cereus
?
panose + H2O
-
326246
Bacillus coagulans
D-glucose + maltose
-
326246
Bacillus coagulans
?
phenyl-alpha-D-glucopyranoside + H2O
-
326246
Bacillus coagulans
D-glucose + phenol
-
326246
Bacillus coagulans
?
sucrose + H2O
-
326246
Bacillus coagulans
D-glucose + D-fructose
-
326246
Bacillus coagulans
?
turanose + H2O
-
326246
Bacillus coagulans
D-glucose + D-fructose
-
326246
Bacillus coagulans
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
62
-
-
Bacillus coagulans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
47
75
half-optima at 47°C and 75°C, no activity at 78°C
Bacillus coagulans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
53
-
stable at pH 6.8 for 10 min
Bacillus coagulans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
-
Bacillus coagulans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.1
10.2
half-optima at pH 4.8 and 7.3
Bacillus coagulans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9.1
50% inactivation at pH 5.0 and 9.1 after incubation at 34°C for 15 h
Bacillus coagulans
6.5
7.3
50% inactivation at pH 6.5 and 7.3 after incubation at 34°C for 15 h
Bacillus cereus
Other publictions for EC 3.2.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750760
Wang
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8
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1
1
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1
1
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1
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1
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1
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1
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8
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1
1
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2
2
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750849
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1
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1
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12
2
1
1
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1
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1
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2
1
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1
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12
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-
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1
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2
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1
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1
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1
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11
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1
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3
2
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1
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1
-
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2
-
1
-
-
-
1
-
-
-
-
5
5
-
-
-
729378
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Rattus norvegicus
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24
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-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
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3
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3
3
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1
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-
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729747
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-
-
-
-
2
-
5
21
-
-
-
-
-
4
-
-
-
-
-
-
21
-
27
-
3
-
14
21
3
-
-
-
10
-
-
-
-
-
-
-
2
-
-
17
14
22
-
-
-
-
-
-
-
-
-
-
21
-
27
-
3
-
20
22
3
-
-
-
-
-
-
-
21
22
725283
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Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639
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195
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-
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1
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1
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3
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2
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1
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2
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1
4
1
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3
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1
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1
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1
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3
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1
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2
-
1
4
1
-
-
3
-
-
-
-
1
1
-
-
1
-
-
729812
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22
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1
-
-
4
-
-
-
-
-
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1
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1
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4
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-
-
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-
-
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730248
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Maltase-glucoamylase modulates ...
Mus musculus
J. Pediatr. Gastroenterol. Nutr.
57
704-712
2013
-
1
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-
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1
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714471
Jones
Mapping the intestinal alpha-g ...
Homo sapiens
Bioorg. Med. Chem.
19
3929-3934
2011
-
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1
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-
-
7
-
-
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1
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1
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1
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1
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6
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1
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7
6
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-
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-
-
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1
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1
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1
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-
-
-
-
-
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-
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-
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717357
Eskandari
Selectivity of 3-O-methylponko ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
6491-6494
2011
-
1
-
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-
10
-
1
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1
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2
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4
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6
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1
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6
10
4
-
1
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2
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704667
Sim
Structural basis for substrate ...
Homo sapiens
J. Biol. Chem.
285
17763-17770
2010
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1
1
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3
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1
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1
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3
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1
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1
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2
2
3
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1
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1
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1
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4
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3
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3
3
717588
Yamamoto
Crystal structures of isomalta ...
Saccharomyces cerevisiae
FEBS J.
277
4205-4214
2010
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1
1
1
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1
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1
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1
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1
1
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717798
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656123
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Watanabe
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655196
Oslancova
Oligo-1,6-glucosidase and neop ...
Bacillus cereus, Bacillus coagulans, Bacillus flavocaldarius, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) F5, Bacillus subtilis, Erwinia rhapontici, Parageobacillus thermoglucosidasius
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Substrate recognition by three ...
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Comparison of sucrase-free iso ...
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2001
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Watanabe
Identification of catalytic an ...
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1
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326268
Wiltz
Expression of enzymatically ac ...
Homo sapiens
Gastroenterology
100
1266-1278
1991
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1
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1
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136512
Galand
First purification and charact ...
Anser anser
Biochim. Biophys. Acta
1033
35-40
1990
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1
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1
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1
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136513
Beaulieu
Detection and characterization ...
Homo sapiens
Gastroenterology
98
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1990
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1
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326261
Yamamoto
Purification and properties of ...
Bacillus sp. F5
Carbohydr. Res.
197
227-235
1990
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-
-
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7
7
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1
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7
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1
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1
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1
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7
-
7
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2
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1
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1
-
7
-
1
-
1
-
1
-
1
-
-
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-
-
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326266
Broyart
Molecular cloning and characte ...
Rattus norvegicus
Biochim. Biophys. Acta
1087
61-67
1990
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2
-
-
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326241
Watanabe
Cloning and expression in Esch ...
Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
J. Bacteriol.
171
1219-1222
1989
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1
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1
-
8
-
1
-
1
-
1
-
-
-
-
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326242
Plant
A cell-associated oligo-1,6-al ...
Thermoanaerobium sp., Thermoanaerobium sp. Tok6-B1
Biochem. J.
255
865-868
1988
2
-
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8
1
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1
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1
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12
-
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2
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1
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2
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8
-
1
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3
1
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1
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1
-
12
-
-
-
2
-
-
1
-
-
-
-
-
-
-
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326243
Lecommandeur
-
Purification of germinated bar ...
Hordeum vulgare
J. Chromatogr.
441
436-442
1988
-
-
-
-
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-
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1
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1
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1
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1
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1
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-
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326244
Suzuki
-
A strong correlation between t ...
Bacillus cereus, Bacillus coagulans, Bacillus flavocaldarius, Bacillus thermoamyloliquefaciens KP1071, Parageobacillus thermoglucosidasius
Appl. Microbiol. Biotechnol.
26
546-551
1987
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-
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4
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5
-
4
5
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1
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5
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1
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8
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1
1
4
-
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4
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5
-
4
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1
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5
-
1
-
8
-
1
1
4
-
-
-
-
-
-
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326245
Kakefuda
Chloroplast and extrachloropla ...
Pisum sativum
Planta
168
175-182
1986
-
-
-
-
-
-
-
-
1
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1
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1
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1
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1
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2
-
1
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-
-
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-
-
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-
-
-
-
-
326246
Suzuki
Purification and characterizat ...
Bacillus cereus, Bacillus coagulans
Eur. J. Biochem.
158
77-83
1986
-
-
-
-
-
-
35
6
-
-
-
-
2
5
-
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1
-
-
-
-
-
11
-
1
1
1
-
1
1
2
-
-
-
-
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-
-
-
-
-
-
-
35
-
6
-
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2
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1
-
-
-
-
11
-
1
1
1
-
1
1
2
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-
-
-
-
-
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326247
Yoshigi
-
Production of alpha-glucosidas ...
Bacillus cereus, Bacillus cereus NY-14
Agric. Biol. Chem.
50
1335-1337
1986
-
-
-
-
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2
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4
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-
4
-
-
-
-
-
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-
-
-
-
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-
-
-
-
326248
Suzuki
-
The lack of identity between B ...
Geobacillus stearothermophilus, Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
Appl. Microbiol. Biotechnol.
20
218-220
1984
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7
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3
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3
-
-
-
-
-
-
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-
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326249
Wacker
A two-active site one-polypept ...
Callorhinus ursinus, Zalophus californianus
J. Biol. Chem.
259
4878-4884
1984
-
-
-
-
-
-
2
3
1
-
1
-
-
6
-
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2
2
-
2
1
-
8
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
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2
-
3
1
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1
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2
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2
1
-
8
1
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-
-
-
1
-
1
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-
-
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326250
Suzuki
-
A simplified method of the pur ...
Bacillus cereus
Eur. J. Appl. Microbiol. Biotechnol.
18
254-256
1983
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-
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1
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1
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1
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1
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1
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1
-
1
-
-
-
-
-
-
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-
-
-
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326251
Suzuki
-
Assignment of a p-nitrophenyl- ...
Bacillus cereus
Biochim. Biophys. Acta
704
476-483
1982
-
-
-
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16
7
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1
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1
1
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1
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1
6
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1
1
1
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1
1
1
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16
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7
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1
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1
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1
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1
6
-
1
1
1
-
1
1
1
-
-
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-
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326252
Suzuki
Abnormally high tolerance agai ...
Bacillus cereus, Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
Biochim. Biophys. Acta
705
124-126
1982
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8
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3
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8
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3
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136508
Sjöström
A fully active, two-active-sit ...
Sus scrofa
J. Biol. Chem.
255
11332-11338
1980
-
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3
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3
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1
1
1
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1
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2
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3
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3
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1
1
-
1
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-
2
-
-
-
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136506
Hauri
Biogenesis of intestinal plasm ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
76
5183-5186
1979
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1
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1
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3
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326253
Skovbjerg
Does sucrase-isomaltase always ...
Homo sapiens
FEBS Lett.
108
399-402
1979
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1
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3
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326254
Suzuki
Hydrolysis of low molecular we ...
Parageobacillus thermoglucosidasius, Parageobacillus thermoglucosidasius KP 1006
Biochim. Biophys. Acta
566
62-66
1979
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1
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12
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1
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6
1
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1
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12
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1
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326255
Marshall
Solubilization of porcine inte ...
Sus scrofa
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82
435-444
1977
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1
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4
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326257
Quaroni
Affinity labeling of the activ ...
Oryctolagus cuniculus
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249
6424-6433
1974
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326258
Asp
Separation of human small-inte ...
Homo sapiens
FEBS Lett.
35
303-305
1973
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393312
Khan
Purification and characterizat ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
146
173-180
1967
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1
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1
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