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Literature summary for 3.2.1.1 extracted from
- Isolation and characterization of a cold-active, alkaline, detergent stable alpha-amylase from a novel bacterium Bacillus subtilis N8 (2018), Prep. Biochem. Biotechnol., 48, 419-426 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | poor effect of 2-mercaptoethanol | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Bacillus subtilis |  |
| Ca2+ | - |
Bacillus subtilis | |
| EDTA | - |
Bacillus subtilis | |
| H2O2 | - |
Bacillus subtilis | |
| Mn2+ | - |
Bacillus subtilis | |
| Na+ | - |
Bacillus subtilis | |
| SDS | - |
Bacillus subtilis | |
| Triton X-100 | - |
Bacillus subtilis | |
| Urea | - |
Bacillus subtilis | |
| Zn2+ | - |
Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | activates | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 205000 | - |
native PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Bacillus subtilis | - |
D-glucose + maltose + maltotriose | - |
? | |
| starch + H2O | Bacillus subtilis N8 | - |
D-glucose + maltose + maltotriose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isolated from soil samples | - |
| Bacillus subtilis N8 | - |
isolated from soil samples | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | amylase synthesis in strain N8 optimally occurs at 15°C and pH 10.0 on agar plates containing starch. Optimization of growth conditions, overview | Bacillus subtilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Bacillus subtilis | D-glucose + maltose + maltotriose | - |
? | |
| starch + H2O | - |
Bacillus subtilis N8 | D-glucose + maltose + maltotriose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 205000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cold-active alpha-amylase | - |
Bacillus subtilis |
| endo-1,4-alpha-D-glucanohydrolase | - |
Bacillus subtilis |
| N8 alpha-amylase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | activity range | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
56% remaining activity after 24 h at pH 8.0, 50% after 24 h at pH 11.0-12.0 | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
56% remaining activity after 24 h at 25°C | Bacillus subtilis |
| 11 | 12 | 50% remaining activity after 24 h at 25°C | Bacillus subtilis |
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