Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Emampour, M.; Noghabi, K.; Zahiri, H.
    Molecular cloning and biochemical characterization of a novel cold-adapted alpha-amylase with multiple extremozyme characteristics (2015), J. Mol. Catal. B, 111, 79-86 .
No PubMed abstract available
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
glycerol activates 15% at 20% v/v, and 27% at 50% v/v Exiguobacterium sp. SH3
additional information no or poor effects by glucose, sucrose, maltose, mannitol, trehalose, sorbitol, myo-inositol, and iodoacetate at 5-10 mM Exiguobacterium sp. SH3
Triton X-100 activates 33% at 20% v/v, and 46% at 50% v/v Exiguobacterium sp. SH3
Tween 20 activates 27% at 20% v/v, and 39% at 50% v/v Exiguobacterium sp. SH3

Cloned(Commentary)

Cloned (Comment) Organism
gene amy-E, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Exiguobacterium sp. SH3

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 slight inhibition at 5-10 mM Exiguobacterium sp. SH3
acetone inhibits 34% at 20% v/v, and 61% at 50% v/v Exiguobacterium sp. SH3
butanol inhibits 15% at 20% v/v, and 21% at 50% v/v Exiguobacterium sp. SH3
DMSO inhibits 20% at 20% v/v, and 31% at 50% v/v Exiguobacterium sp. SH3
EDTA slight inhibition at 5-10 mM Exiguobacterium sp. SH3
ethanol inhibits 9% at 20% v/v, and 18% at 50% v/v Exiguobacterium sp. SH3
guanidinium hydrochloride slight inhibition at 5-10 mM Exiguobacterium sp. SH3
methanol inhibits 7% at 20% v/v, and 13% at 50% v/v Exiguobacterium sp. SH3
additional information no or poor effects by glucose, sucrose, maltose, mannitol, trehalose, sorbitol, myo-inositol, and iodoacetate at 5-10 mM Exiguobacterium sp. SH3
SDS slight inhibition at 5-10 mM Exiguobacterium sp. SH3
Urea slight inhibition at 5-10 mM Exiguobacterium sp. SH3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km of soluble starch hydrolysis is 2.29 mg/ml, thermodynamics, overview Exiguobacterium sp. SH3

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme has a signal peptide of 25 amino acids at the N-terminus Exiguobacterium sp. SH3
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
K+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
Li+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
Mg2+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
additional information no or poor effects by 5-10 mM of Mn2+, Cu2+, Zn2+, and Fe2+ Exiguobacterium sp. SH3
Na+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
NaCl Amy-E not only is halotolerant but also its activity is stimulated at high salt concentrations in the range of 1-5 M, activates 20% at 2 M Exiguobacterium sp. SH3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 about, native PAGE, recombinant enzyme Exiguobacterium sp. SH3

Organism

Organism UniProt Comment Textmining
Exiguobacterium sp. SH3 A0A0A0WA73 isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Exiguobacterium sp. SH3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 62.2% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?
maltodextrin + H2O 87.6% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?
rice flour + H2O 48.7% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?
soluble starch + H2O best substrate Exiguobacterium sp. SH3 ?
-
 ?
wheat flour + H2O 53.0% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 53000, recombinant His-tagged enzyme, SDS-PAGE Exiguobacterium sp. SH3

Synonyms

Synonyms Comment Organism
Amy-E
-
 Exiguobacterium sp. SH3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Exiguobacterium sp. SH3

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 80 41% of maximal activity at 0°C, 63%, 50%, 71%, 48%, 39%, 26%, and 14% at 20°C, 10°C, 40°C, 50°C, 60°C, 70°C, and 80°C, respectively Exiguobacterium sp. SH3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 60 purified recombinant His-tagged enzyme, stable up to 30°C, the enzyme activity drops to 82%, 39%, and 16% after5 min at 40°C, 50°C, and 60°C, and to 41%, 15%, and 4% after 30 min, respectively Exiguobacterium sp. SH3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant enzyme Exiguobacterium sp. SH3

pH Range

pH Minimum pH Maximum Comment Organism
4 9 activity range, profile overview Exiguobacterium sp. SH3

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 13, GH13 Exiguobacterium sp. SH3
additional information the catalytic triad is formed by residues D210,E239, and D307 Exiguobacterium sp. SH3