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Literature summary for 3.2.1.1 extracted from

  • Allala, F.; Bouacem, K.; Boucherba, N.; Azzouz, Z.; Mechri, S.; Sahnoun, M.; Benallaoua, S.; Hacene, H.; Jaouadi, B.; Bouanane-Darenfed, A.
    Purification, biochemical, and molecular characterization of a novel extracellular thermostable and alkaline alpha-amylase from Tepidimonas fonticaldi strain HB23 (2019), Int. J. Biol. Macromol., 132, 558-574 .
    View publication on PubMed
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
chloroform activates by about 40% at 25% v/v Tepidimonas fonticaldi
Cyclohexane activates by about 15% at 25% v/v Tepidimonas fonticaldi
Dimethyl formamide activates by about 55% at 25% v/v Tepidimonas fonticaldi
DMSO activates by 10% at 25% v/v Tepidimonas fonticaldi
ethanol activates by 15% at 25% v/v Tepidimonas fonticaldi
n-hexane activates by about 13% at 25% v/v Tepidimonas fonticaldi

Cloned(Commentary)

Cloned (Comment) Organism
gene amy48, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of the extracellular fraction of enzyme TfAmy48 in Escherichia coli strain BL21(DE3)pLysS. The biochemical properties of the extracellular purified recombinant enzyme (rTfAmy48) are similar to those of the native one Tepidimonas fonticaldi

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 59% inhibition at 10 mM Tepidimonas fonticaldi
4-bromophenacyl bromide 19% inhibition at 2 mM Tepidimonas fonticaldi
Ag+ inhibits 24% at 5 mM Tepidimonas fonticaldi
Cd2+ complete inhibition at 5 mM Tepidimonas fonticaldi
Co2+ inhibits 60% at 5 mM Tepidimonas fonticaldi
Cu2+ inhibits 75% at 5 mM Tepidimonas fonticaldi
DTT 74% inhibition at 1 mM Tepidimonas fonticaldi
Hg2+ complete inhibition at 5 mM Tepidimonas fonticaldi
Mn2+ inhibits 11% at 5 mM Tepidimonas fonticaldi
additional information enzyme TfAmy48 shows excellent compatibility with some commercial laundry detergents, overview. No or poor effects by 1 mM PMSF, 2 mM diiodopropyl fluorophosphate, 10 mM EDTA, or 1 mm EGTA Tepidimonas fonticaldi
SDS 39% inhibition at 60 mM Tepidimonas fonticaldi
Urea 51% inhibition at 6 M Tepidimonas fonticaldi
Zn2+ inhibits 79% at 5 mM Tepidimonas fonticaldi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information classical Michaelis-Menten kinetics, Km for soluble potato starch is 1.22 mg/ml Tepidimonas fonticaldi

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme harbors a predicted signal peptide Tepidimonas fonticaldi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates 38% at 5 mM Tepidimonas fonticaldi
Ca2+ activates 55% at 5 mM Tepidimonas fonticaldi
Fe2+ activates 22% at 5 mM Tepidimonas fonticaldi
Mg2+ activates 13% at 5 mM Tepidimonas fonticaldi
Ni2+ activates 19% at 5 mM Tepidimonas fonticaldi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 native PAGE and gel filtration Tepidimonas fonticaldi
48138
-
 mass spectrometry Tepidimonas fonticaldi

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 75% inhibition at 25% v/v Tepidimonas fonticaldi
benzene 55% inhibition at 25% v/v Tepidimonas fonticaldi
Ethyl acetate 55% inhibition at 25% v/v Tepidimonas fonticaldi
isooctane 15% inhibition at 25% v/v Tepidimonas fonticaldi
additional information enzyme TfAmy48 shows extreme stability and tolerance towards organic solvents. After 24 h, TfAmy48 shows a stimulatory effect in the majority of hydrophilic organic solvents, including DMSO, ethanol, chloroform, and DMF with a residual activity of 110, 115, 140, and 155%, respectively. No of poor effects on enzyme activity by 25% v/v of isopropyl alcohol, isopropanol, n-decane, toluene, n-hexanol, n-butanol, and methanol Tepidimonas fonticaldi
n-heptane 25% inhibition at 25% v/v Tepidimonas fonticaldi
n-octane 10% inhibition at 25% v/v Tepidimonas fonticaldi

Organism

Organism UniProt Comment Textmining
Tepidimonas fonticaldi A0A482EWM9 isolated from a collection obtained froma thermal spring in Hammam Righa commune (GPS coordinates: 36°22' N, 2°23' E), located in Ain Defla province (Algeria). The isolation site is located at an altitude of 525 m, in a mountainous and wooded area. It includes several sources whose water temperature varies from 26°C to 68°C
-
Tepidimonas fonticaldi HB23 A0A482EWM9 isolated from a collection obtained froma thermal spring in Hammam Righa commune (GPS coordinates: 36°22' N, 2°23' E), located in Ain Defla province (Algeria). The isolation site is located at an altitude of 525 m, in a mountainous and wooded area. It includes several sources whose water temperature varies from 26°C to 68°C
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 9.5fold by ammonium sulfate fractionation, heat treatment, gel filtration, and anion exchange chromatography Tepidimonas fonticaldi

Source Tissue

Source Tissue Comment Organism Textmining
additional information culture growth in optimized starch broth Tepidimonas fonticaldi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1275
-
 purified native extracellular enzyme, substrate soluble starch, pH 8.0, 80°C Tepidimonas fonticaldi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 75% activity compared to soluble potato starch Tepidimonas fonticaldi ?
-
 ?
amylopectin + H2O 75% activity compared to soluble potato starch Tepidimonas fonticaldi HB23 ?
-
 ?
amylose + H2O 85% activity compared to soluble potato starch Tepidimonas fonticaldi ?
-
 ?
amylose + H2O 85% activity compared to soluble potato starch Tepidimonas fonticaldi HB23 ?
-
 ?
additional information enzyme TfAmy48 shows an endo-acting pattern. It has no activity with raw starch, alpha-cyclodextrin, and carboxymethyl cellulose Tepidimonas fonticaldi ?
-
 ?
additional information enzyme TfAmy48 shows an endo-acting pattern. It has no activity with raw starch, alpha-cyclodextrin, and carboxymethyl cellulose Tepidimonas fonticaldi HB23 ?
-
 ?
soluble maize starch + H2O 60% activity compared to soluble potato starch Tepidimonas fonticaldi ?
-
 ?
soluble maize starch + H2O 60% activity compared to soluble potato starch Tepidimonas fonticaldi HB23 ?
-
 ?
soluble potato starch + H2O best substrate Tepidimonas fonticaldi maltopentaose + maltose + maltotriose
-
 ?
soluble potato starch + H2O best substrate Tepidimonas fonticaldi HB23 maltopentaose + maltose + maltotriose
-
 ?
soluble rice starch + H2O 55% activity compared to soluble potato starch Tepidimonas fonticaldi ?
-
 ?
soluble wheat starch + H2O 79% activity compared to soluble potato starch Tepidimonas fonticaldi ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48138, mass spectrometry, 1 * 48000, SDS-PAGE, 1 * 50414, sequence calculation Tepidimonas fonticaldi
More enzyme three-dimensional structure homology modelling, overview Tepidimonas fonticaldi

Synonyms

Synonyms Comment Organism
TfAmy48
-
 Tepidimonas fonticaldi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 75 without Ca2+ Tepidimonas fonticaldi
80
-
 in presence of 5 mM Ca2+ Tepidimonas fonticaldi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified enzyme, 5 mM Ca2+, completely stable for 14 h, loss of 50% activity after 19 h, inactivation after 23 h Tepidimonas fonticaldi
70
-
 purified enzyme, without Ca2+, completely stable for 10 h, loss of 50% activity after 16 h, inactivation after 19 h Tepidimonas fonticaldi
80
-
 purified enzyme, 5 mM Ca2+, completely stable for 8 h, loss of 50% activity after 13 h, inactivation after 16 h Tepidimonas fonticaldi
80
-
 purified enzyme, without Ca2+, completely stable for 6 h, loss of 50% activity after 10 h, inactivation after 13 h Tepidimonas fonticaldi
90
-
 purified enzyme, 5 mM Ca2+, completely stable for 5 h, loss of 50% activity after 8 h, inactivation after 10 h Tepidimonas fonticaldi
90
-
 purified enzyme, without Ca2+, completely stable for 3 h, loss of 50% activity after 6 h, inactivation after 8 h Tepidimonas fonticaldi
100
-
 purified enzyme, 5 mM Ca2+, completely stable for 1 h, loss of 50% activity after 3 h, inactivation after 4 h Tepidimonas fonticaldi
100
-
 purified enzyme, without Ca2+, loss of 50% activity after 2 h, inactivation after 3h Tepidimonas fonticaldi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.9
-
 soluble potato starch pH 8.0, 80°C Tepidimonas fonticaldi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Tepidimonas fonticaldi

pH Range

pH Minimum pH Maximum Comment Organism
4 12 over 50% of maximal activity within this range, profile overview Tepidimonas fonticaldi

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 purified enzyme, completely stable for 16 h, loss of 50% activity after 21 h, inactivation after 24 h Tepidimonas fonticaldi
7
-
 purified enzyme, completely stable for 12.5 h, loss of 50% activity after 18 h, inactivation after 21 h Tepidimonas fonticaldi
8
-
 purified enzyme, completely stable for 9 h, loss of 50% activity after 15 h, inactivation after 18 h Tepidimonas fonticaldi
9
-
 purified enzyme, completely stable for 8 h, loss of 50% activity after 11 h, inactivation after 13 h Tepidimonas fonticaldi
10
-
 purified enzyme, completely stable for 6 h, loss of 50% activity after 9 h, inactivation after 11 h Tepidimonas fonticaldi
11
-
 purified enzyme, completely stable for 3 h, loss of 50% activity after 6 h, inactivation after 8 h Tepidimonas fonticaldi

pI Value

Organism Comment pI Value Maximum pI Value
Tepidimonas fonticaldi sequence calculation
-
 6.04

General Information

General Information Comment Organism
additional information enzyme three-dimensional structure homology modelling, overview Tepidimonas fonticaldi