Literature summary for 3.2.1.1 extracted from

Acer, O.e.; Bekler, F.M.; Pirinccioglu, H.; Gueven, R.G.; Gueven, K.
Purification and characterization of thermostable and detergent-stable alpha-amylase from Anoxybacillus sp. AH1 (2016), Food Technol. Biotechnol., 54, 70-77 .

Search for more literature for 3.2.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	64% activation at 10 mM	Anoxybacillus sp. AH1
DTT	106% activation at 10 mM	Anoxybacillus sp. AH1
additional information	the enzyme activity is increased by various detergents	Anoxybacillus sp. AH1
SDS	28% activation at 0.5%	Anoxybacillus sp. AH1
Triton X-100	26% activation at 0.5%	Anoxybacillus sp. AH1
Tween 40	38% activation at 0.5%	Anoxybacillus sp. AH1

General Stability

General Stability	Organism
the purified enzyme is highly stable in the presence of 30% glycerol	Anoxybacillus sp. AH1

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	22% inhibition at 10 mM	Anoxybacillus sp. AH1
4-chloromercuribenzoic acid	52% inhibition at 4 mM	Anoxybacillus sp. AH1
Cu2+	76% inhibition at 0.5 mM, complete inhibition at 1 mM	Anoxybacillus sp. AH1
EDTA	63% inhibition at 10 mM	Anoxybacillus sp. AH1
iodoacetamide	slight inhibition	Anoxybacillus sp. AH1
N-ethylmaleimide	slight inhibition	Anoxybacillus sp. AH1
phenylmethylsulfonyl fluoride	60% inhibition at 4 mM	Anoxybacillus sp. AH1
Urea	91% inhibition at 2 mM	Anoxybacillus sp. AH1
Zn2+	93% inhibition at 1 mM	Anoxybacillus sp. AH1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Lineweaver-Burk plot, Michaelis-Menten kinetics	Anoxybacillus sp. AH1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Anoxybacillus sp. AH1
Mg2+	activates	Anoxybacillus sp. AH1

Organism

Organism	UniProt	Comment	Textmining
Anoxybacillus sp. AH1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 18fold by dialysis, gel filtration, and ultrafiltration	Anoxybacillus sp. AH1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	highest production activity of 1874.8 U/ml obtained at 24 h of incubation	Anoxybacillus sp. AH1
27065	-	the purified enzyme, one unit of amylase activity is defined as the amount of enzyme that released 1 micromol of maltose per minute per ml under the assay conditions, pH 7.0, 60°C	Anoxybacillus sp. AH1

Subunits

Subunits	Comment	Organism
?	x * 85000, SDS-PAGE	Anoxybacillus sp. AH1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Anoxybacillus sp. AH1

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	80	activity range, profile overview	Anoxybacillus sp. AH1

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	purified enzyme, 30% glycerol, 85% activity remaining at 60°C after 2 h, 93% after 1 h. Without glycerol, the enzyme loses about 30% activity after 2 h	Anoxybacillus sp. AH1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Anoxybacillus sp. AH1

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	11	activity range, profile overview. Over 80% of maximal activity at pH 6.5-8.0	Anoxybacillus sp. AH1

General Information

General Information	Comment	Organism
additional information	at least one essential cysteine residue is located in the active site of the enzyme	Anoxybacillus sp. AH1

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)