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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of alpha-amylase from Trichoderma pseudokoningii (2018), BMC Biochem., 19, 4 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | strong inhibition | Trichoderma pseudokoningii |  |
| Co2+ | 28% inhibition at 5 mM | Trichoderma pseudokoningii | |
| Cu2+ | 25% inhibition at 5 mM | Trichoderma pseudokoningii | |
| EDTA | strong inhibition | Trichoderma pseudokoningii | |
| Fe2+ | - |
Trichoderma pseudokoningii | |
| Hg2+ | 62% inhibition at 5 mM | Trichoderma pseudokoningii | |
| Pb2+ | 20% inhibition at 5 mM | Trichoderma pseudokoningii | |
| Zn2+ | 35% inhibition at 5 mM | Trichoderma pseudokoningii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km and Vmax values of A4 are 4 mg starch/ml and 0.00074 mmol reducing sugar, respectively | Trichoderma pseudokoningii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma pseudokoningii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates 52% at 5 mM | Trichoderma pseudokoningii | |
| Mg2+ | activates 34% at 5 mM | Trichoderma pseudokoningii | |
| additional information | no effect by 5 mM Ni2+ | Trichoderma pseudokoningii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
native enzyme, gel filtration | Trichoderma pseudokoningii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Trichoderma pseudokoningii | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma pseudokoningii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 15.7fold by anion exchange chromatography, to homogeneity | Trichoderma pseudokoningii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | grown on orange peel under solid state fermentation | Trichoderma pseudokoningii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | 13% activity compared to starch | Trichoderma pseudokoningii | ? | - |
? | |
| amylopectin + H2O | 73% activity compared to starch | Trichoderma pseudokoningii | ? | - |
? | |
| amylose + H2O | 55% activity compared to starch | Trichoderma pseudokoningii | ? | - |
? | |
| beta-cyclodextrin + H2O | 12% activity compared to starch | Trichoderma pseudokoningii | ? | - |
? | |
| glycogen + H2O | 85% activity compared to starch | Trichoderma pseudokoningii | ? | - |
? | |
| starch + H2O | - |
Trichoderma pseudokoningii | ? | - |
? | |
| starch + H2O | best substrate, potato starch | Trichoderma pseudokoningii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Trichoderma pseudokoningii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-amylase A4 | - |
Trichoderma pseudokoningii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trichoderma pseudokoningii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | activity range, profile overview | Trichoderma pseudokoningii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, 1 h, completely stable up to | Trichoderma pseudokoningii |
| 60 | - |
purified enzyme, 1 h, about 80% activity remaining | Trichoderma pseudokoningii |
| 80 | - |
purified enzyme, 1 h, about 70% activity remaining | Trichoderma pseudokoningii |
| 90 | - |
purified enzyme, 1 h, about 20% activity remaining | Trichoderma pseudokoningii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Trichoderma pseudokoningii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | over 60% of maximal activity within this range | Trichoderma pseudokoningii |
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