Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Corallococcus sp. EGB |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Corallococcus sp. EGB |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
amylopectin | Km-value: 10.3 mg/ml, pH 7.0, 50°C | Corallococcus sp. EGB | |
additional information | - |
starch | Km-value: 2.9 mg/ml, pH 7.0, 50°C | Corallococcus sp. EGB |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59946 | - |
calculated from sequence | Corallococcus sp. EGB |
60000 | - |
SDS-PAGE | Corallococcus sp. EGB |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corallococcus sp. EGB | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corallococcus sp. EGB |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
121.3 | - |
substrate: amylopectin, pH 7.0, 50°C | Corallococcus sp. EGB |
980 | - |
substrate: starch, pH 7.0, 50°C | Corallococcus sp. EGB |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
gamma-cyclodextrin + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
maltoheptaose + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
maltohexaose + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
maltotetraose + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
maltotriose + H2O | no activity against isomaltotriose (alpha-glucosyl-(1->6)-alpha-glucosyl-(1->6)-glucose) | Corallococcus sp. EGB | maltose + ? | - |
? | |
additional information | the enzyme hydrolyses alpha-1,4-glycosidic bonds and not alpha-1,6-linkages. It has no activity toward maltose, alpha-cyclodextrin, beta-cyclodextrin, panose, acarbose and dextran | Corallococcus sp. EGB | ? | - |
? | |
starch + H2O | action mode is endolytic, hydrolysis products have an alpha-anomeric configuration. Degradation of 1% (w/v) starch results in the formation of oligosaccharides in the maltose to maltopentaose range at early time points, followed by an accumulation of maltose, maltotetraose, and small amounts of glucose after 1 h, and its final end products consisted of a high level of 58% (w/w) maltose without concomitant production of glucose | Corallococcus sp. EGB | alpha-maltose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ComA | - |
Corallococcus sp. EGB |
maltogenic amylase | - |
Corallococcus sp. EGB |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Corallococcus sp. EGB |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | 25°C: about 45% of maximal activity, 60°C: about 45% of maximal activity | Corallococcus sp. EGB |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
almost all of the enzyme activity remains after the enzyme is incubated for 60 min at temperatures of below 40°C | Corallococcus sp. EGB |
50 | - |
30 min, not stable above 50°C | Corallococcus sp. EGB |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2274.8 | - |
amylopectin | pH 7.0, 50°C | Corallococcus sp. EGB | |
3021.2 | - |
starch | pH 7.0, 50°C | Corallococcus sp. EGB |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Corallococcus sp. EGB |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 80% of maximal activity, pH 9.0: about 45% of maximal activity | Corallococcus sp. EGB |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 4°C, 24 h, enzyme retains more than 50% activity | Corallococcus sp. EGB |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corallococcus sp. EGB | calculated from sequence | - |
6.08 |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme may be involved in the accumulation of maltose in Corallococcus media | Corallococcus sp. EGB |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
220 | - |
amylopectin | pH 7.0, 50°C | Corallococcus sp. EGB | |
1019.6 | - |
starch | pH 7.0, 50°C | Corallococcus sp. EGB |