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Literature summary for 3.2.1.1 extracted from

  • Zhou, J.; Li, Z.; Zhang, H.; Wu, J.; Ye, X.; Dong, W.; Jiang, M.; Huang, Y.; Cui, Z.
    Novel maltogenic amylase CoMA from Corallococcus sp. strain EGB catalyzes the conversion of maltooligosaccharides and soluble starch to maltose (2018), Appl. Environ. Microbiol., 84, e00152-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corallococcus sp. EGB

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
Corallococcus sp. EGB

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
amylopectin Km-value: 10.3 mg/ml, pH 7.0, 50°C Corallococcus sp. EGB
additional information
-
starch Km-value: 2.9 mg/ml, pH 7.0, 50°C Corallococcus sp. EGB

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59946
-
calculated from sequence Corallococcus sp. EGB
60000
-
SDS-PAGE Corallococcus sp. EGB

Organism

Organism UniProt Comment Textmining
Corallococcus sp. EGB
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Corallococcus sp. EGB

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
121.3
-
substrate: amylopectin, pH 7.0, 50°C Corallococcus sp. EGB
980
-
substrate: starch, pH 7.0, 50°C Corallococcus sp. EGB

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Corallococcus sp. EGB maltose + ?
-
?
gamma-cyclodextrin + H2O
-
Corallococcus sp. EGB maltose + ?
-
?
maltoheptaose + H2O
-
Corallococcus sp. EGB maltose + ?
-
?
maltohexaose + H2O
-
Corallococcus sp. EGB maltose + ?
-
?
maltotetraose + H2O
-
Corallococcus sp. EGB maltose + ?
-
?
maltotriose + H2O no activity against isomaltotriose (alpha-glucosyl-(1->6)-alpha-glucosyl-(1->6)-glucose) Corallococcus sp. EGB maltose + ?
-
?
additional information the enzyme hydrolyses alpha-1,4-glycosidic bonds and not alpha-1,6-linkages. It has no activity toward maltose, alpha-cyclodextrin, beta-cyclodextrin, panose, acarbose and dextran Corallococcus sp. EGB ?
-
?
starch + H2O action mode is endolytic, hydrolysis products have an alpha-anomeric configuration. Degradation of 1% (w/v) starch results in the formation of oligosaccharides in the maltose to maltopentaose range at early time points, followed by an accumulation of maltose, maltotetraose, and small amounts of glucose after 1 h, and its final end products consisted of a high level of 58% (w/w) maltose without concomitant production of glucose Corallococcus sp. EGB alpha-maltose + ?
-
?

Synonyms

Synonyms Comment Organism
ComA
-
Corallococcus sp. EGB
maltogenic amylase
-
Corallococcus sp. EGB

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Corallococcus sp. EGB

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 25°C: about 45% of maximal activity, 60°C: about 45% of maximal activity Corallococcus sp. EGB

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
almost all of the enzyme activity remains after the enzyme is incubated for 60 min at temperatures of below 40°C Corallococcus sp. EGB
50
-
30 min, not stable above 50°C Corallococcus sp. EGB

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2274.8
-
amylopectin pH 7.0, 50°C Corallococcus sp. EGB
3021.2
-
starch pH 7.0, 50°C Corallococcus sp. EGB

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Corallococcus sp. EGB

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 80% of maximal activity, pH 9.0: about 45% of maximal activity Corallococcus sp. EGB

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 4°C, 24 h, enzyme retains more than 50% activity Corallococcus sp. EGB

pI Value

Organism Comment pI Value Maximum pI Value
Corallococcus sp. EGB calculated from sequence
-
6.08

General Information

General Information Comment Organism
additional information the enzyme may be involved in the accumulation of maltose in Corallococcus media Corallococcus sp. EGB

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
220
-
amylopectin pH 7.0, 50°C Corallococcus sp. EGB
1019.6
-
starch pH 7.0, 50°C Corallococcus sp. EGB