Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTAB | 27% inhibition | Streptomyces fragilis | |
SDS | 12% inhibition | Streptomyces fragilis | |
Triton X-100 | 18% inhibition | Streptomyces fragilis | |
Tween 20 | 26% inhibition | Streptomyces fragilis | |
Tween 80 | 22% inhibition | Streptomyces fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Vmax value of purified native enzyme is 0.836 mg/ml | Streptomyces fragilis | |
0.000624 | - |
soluble starch | pH 8.0, 55°C | Streptomyces fragilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces fragilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
gel filtration | Streptomyces fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fragilis | - |
- |
- |
Streptomyces fragilis DA7-7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 17.13fold by ammonium sulfate fractionation and gel filtration | Streptomyces fragilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | optimization of alpha-amylase production, optimum fermentation conditions are 3.07% dextrose, 1.085% peptone, pH 6.0, and incubation temperature 27.27°C. The predicted optimum alpha-amylase activity is 991.82 U/ml/min | Streptomyces fragilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200.76 | - |
purified native enzyme, pH 8.0, 55°C, substrate soluble starch | Streptomyces fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Streptomyces fragilis | ? | - |
? | |
soluble starch + H2O | - |
Streptomyces fragilis DA7-7 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Streptomyces fragilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | purified native enzyme, 83% activity remains at 40°C after 180 min, 72% at 80°C, and 100% at 50°C | Streptomyces fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces fragilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 82 and 72% of maximal activity at pH 5.0 and pH 9.0, respectively | Streptomyces fragilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | purified native enzyme, 100% activity remaining when pre-incubated at pH 6.0 and pH 7.0 for 24 h | Streptomyces fragilis |