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Literature summary for 3.2.1.1 extracted from

  • Nithya, K.; Muthukumar, C.; Kadaikunnan, S.; Alharbi, N.S.; Khaled, J.M.; Dhanasekaran, D.
    Purification, characterization, and statistical optimization of a thermostable alpha-amylase from desert actinobacterium Streptomyces fragilis DA7-7 (2017), 3 Biotech, 7, 350 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
CTAB 27% inhibition Streptomyces fragilis
SDS 12% inhibition Streptomyces fragilis
Triton X-100 18% inhibition Streptomyces fragilis
Tween 20 26% inhibition Streptomyces fragilis
Tween 80 22% inhibition Streptomyces fragilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Vmax value of purified native enzyme is 0.836 mg/ml Streptomyces fragilis
0.000624
-
soluble starch pH 8.0, 55°C Streptomyces fragilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces fragilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
gel filtration Streptomyces fragilis

Organism

Organism UniProt Comment Textmining
Streptomyces fragilis
-
-
-
Streptomyces fragilis DA7-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 17.13fold by ammonium sulfate fractionation and gel filtration Streptomyces fragilis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium optimization of alpha-amylase production, optimum fermentation conditions are 3.07% dextrose, 1.085% peptone, pH 6.0, and incubation temperature 27.27°C. The predicted optimum alpha-amylase activity is 991.82 U/ml/min Streptomyces fragilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200.76
-
purified native enzyme, pH 8.0, 55°C, substrate soluble starch Streptomyces fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
Streptomyces fragilis ?
-
?
soluble starch + H2O
-
Streptomyces fragilis DA7-7 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Streptomyces fragilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 purified native enzyme, 83% activity remains at 40°C after 180 min, 72% at 80°C, and 100% at 50°C Streptomyces fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptomyces fragilis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 82 and 72% of maximal activity at pH 5.0 and pH 9.0, respectively Streptomyces fragilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 purified native enzyme, 100% activity remaining when pre-incubated at pH 6.0 and pH 7.0 for 24 h Streptomyces fragilis