synthesis |
use of probiotic Bacillus spores as a matrix for enzyme immobilization by covalent and adsorption methods. The maximum concentration of the alpha-amylase immobilized is 360 microg/1.2 10EE11spores. Maximum activity is achieved at an enzyme concentration of approximately 60 microg/0.4 10EE10 spores, corresponding to an estimated activity of 8000 IU per mg and 1.2 10EE11 spores for covalent immobilization and 85300 IU for the adsorption method. Enzyme immobilization yield is estimated to be 77% and 20.07% for the covalent and adsorption methods, respectively. The alpha-amylase immobilized by both methods, displays improved activity in the basic pH range. The optimum pH for the free enzyme is 5 while it shifts to 8 for the immobilized enzyme. The optimum temperatures for the free and immobilized enzymes are 0C and 0C, respectively. The covalently immobilized alpha-amylase retains 65% of its initial activity, even after 10 times of recycling |
Bacillus licheniformis |