Literature summary for 3.2.1.1 extracted from

Mizutani, K.; Toyoda, M.; Otake, Y.; Yoshioka, S.; Takahashi, N.; Mikami, B.
Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris (2012), Biochim. Biophys. Acta, 1824, 954-962.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Oryzias latipes

Crystallization (Commentary)

Crystallization (Comment)	Organism
His-tagged recombinant protein, to 2.85 A resolution, space group P3221, one protein molecule per asymmetric unit. One bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules. The overall structure is the TIM barrel structure with two additional domains, domain B protruding out of the barrel in the place of the beta3-alpha3 loop and domain C succeeding the catalytic barrel	Oryzias latipes

Crystallization (Comment)

Organism

His-tagged recombinant protein, to 2.85 A resolution, space group P3221, one protein molecule per asymmetric unit. One bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules. The overall structure is the TIM barrel structure with two additional domains, domain B protruding out of the barrel in the place of the beta3-alpha3 loop and domain C succeeding the catalytic barrel

Oryzias latipes

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: NaCl up to 250 mM	Oryzias latipes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	in the crystal structure, one bound calcium ion is found, coordinated to the side chains of Asn100 and Asp167, the main-chains of Arg158 and His201, and three water molecules	Oryzias latipes
chloride	presence of chloride ions is essential for activity. 20 mM NaCl is sufficient for maximum activity. NaCl is not inhibitory below 250 mM	Oryzias latipes

Organism

Organism	UniProt	Comment	Textmining
Oryzias latipes	H2N0D4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
starch + H2O	corn starch is preferred over wheat starch and sweet-potato starch. Potato starch is a poor substrate	Oryzias latipes	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Oryzias latipes

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	melting temperature	Oryzias latipes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Oryzias latipes