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Literature summary for 3.2.1.1 extracted from
- An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment (2015), Appl. Microbiol. Biotechnol., 99, 717-727.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | uncultured organism |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 2 mM, 72% residual activity | uncultured organism |  |
| Bio-tex | liquid commercial detergent, 75% residual activity | uncultured organism | |
| Cu2+ | 2 mM, no residual activity | uncultured organism | |
| EDTA | 1 mM, almost complete loss of activity | uncultured organism | |
| Green Balance | solid commercial detergent, when used with tap water, 51% residual activity | uncultured organism | |
| Mg2+ | 2 mM, 58% residual activity | uncultured organism | |
| sodium dodecylsulfate | 0.1%, almost complete loss of activity | uncultured organism | |
| Zn2+ | 2 mM, no residual activity | uncultured organism | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
starch | Km value is 1.79 ml/ml at 5°C, pH 8.3 and ,328 mg/ml at 15°C, pH 8.3 | uncultured organism | |
| additional information | - |
amylopectin | Km value is 1.9 ml/ml at 5°C, pH 8.3 and 2.5 mg/ml at 15°C, pH 8.3 | uncultured organism | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, 127% of initial activity | uncultured organism | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured organism | A0A060DAC6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | uncultured organism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
uncultured organism | ? | - |
? | |
| amylose + H2O | - |
uncultured organism | ? | - |
? | |
| maltoheptaose + 3 H2O | - |
uncultured organism | 2 maltose + maltotriose | additional product: maltotetraose | ? | |
| maltopentaose + H2O | - |
uncultured organism | maltose + maltotriose | - |
? | |
| maltotetraose + H2O | - |
uncultured organism | 2 maltose | weak activity | ? | |
| additional information | no substrates: granular starch or glycogen, maltose, maltotriose | uncultured organism | ? | - |
? | |
| starch + H2O | - |
uncultured organism | maltose + ? | and larger maltooligosaccharides | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AmyI3C6 | - |
uncultured organism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 15 | - |
uncultured organism |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 1 | - |
more than 70% of maximum activity | uncultured organism |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | - |
or below, 60 min, no loss of activity | uncultured organism |
| 45 | - |
20 min, complete loss of activity | uncultured organism |
| 55 | - |
5 min, complete loss of activity | uncultured organism |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
starch | 5°C, pH 8.3 | uncultured organism | |
| 0.042 | - |
amylopectin | 5°C, pH 8.3 | uncultured organism | |
| 0.15 | - |
amylopectin | 15°C, pH 8.3 | uncultured organism | |
| 0.156 | - |
starch | 15°C, pH 8.3 | uncultured organism | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
uncultured organism |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | 20°C, stable for at least 14 h | uncultured organism |
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Release 2023.1 (January 2023)
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