Activating Compound | Comment | Organism | Structure |
---|---|---|---|
butanol | 20% (v/v), 25°C, pH 8.5, 2 h, 1.3fold activation | Halorubrum xinjiangense | |
Cyclohexane | 20% (v/v), 25°C, pH 8.5, 2 h, 1.8fold activation | Halorubrum xinjiangense | |
decane | 20% (v/v), 25°C, pH 8.5, 2 h, 1.4fold activation | Halorubrum xinjiangense | |
dodecane | 20% (v/v), 25°C, pH 8.5, 2 h, 1.9fold activation | Halorubrum xinjiangense | |
ethanol | 20% (v/v), 25°C, pH 8.5, 2 h, 1.1fold activation | Halorubrum xinjiangense | |
heptane | 20% (v/v), 25°C, pH 8.5, 2 h, 1.1fold activation | Halorubrum xinjiangense | |
hexadecane | 20% (v/v), 25°C, pH 8.5, 2 h, 2.6fold activation | Halorubrum xinjiangense | |
hexane | 20% (v/v), 25°C, pH 8.5, 2 h, 1.2fold activation | Halorubrum xinjiangense | |
n-Octanol | 20% (v/v), 25°C, pH 8.5, 2 h, 1.9fold activation | Halorubrum xinjiangense | |
nonane | 20% (v/v), 25°C, pH 8.5, 2 h, 2.2fold activation | Halorubrum xinjiangense | |
tetradecane | 20% (v/v), 25°C, pH 8.5, 2 h, 2.5fold activation | Halorubrum xinjiangense | |
Toluene | 20% (v/v), 25°C, pH 8.5, 2 h, 1.5fold activation | Halorubrum xinjiangense | |
xylene | 20% (v/v), 25°C, pH 8.5, 2 h, 2fold activation | Halorubrum xinjiangense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 10 mM, 86% loss of activity | Halorubrum xinjiangense | |
Cu2+ | 10 mM, 88% loss of activity | Halorubrum xinjiangense | |
Fe2+ | 10 mM, 90% loss of activity | Halorubrum xinjiangense | |
Fe3+ | 10 mM, 95% loss of activity | Halorubrum xinjiangense | |
Hg2+ | 10 mM, 88% loss of activity | Halorubrum xinjiangense | |
Ni2+ | 10 mM, 58% loss of activity | Halorubrum xinjiangense | |
Zn2+ | 10 mM, 80% loss of activity | Halorubrum xinjiangense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
soluble starch | Km: 3.8 mg/ml, pH 8.5, 70°C | Halorubrum xinjiangense | |
additional information | - |
starch | the KM-value for soluble starch (pH 8.5, 70°C) is 3.8 mg/ml | Halorubrum xinjiangense |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Halorubrum xinjiangense | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 1.4fold activation | Halorubrum xinjiangense | |
Ca2+ | 10 mM, increase of activity to 140% | Halorubrum xinjiangense | |
KCl | maximum amylase activity at 4 M NaCl or 4.5 M KCl, 70°C, and pH 8.5 | Halorubrum xinjiangense | |
KCl | maximum amylase activity at 4 M NaCl or 4.5 M KCl, and retains about 6090% of the optimal activity at 23.5 M. The enzyme is less active in the presence of 0.51.5 M of each salt, and no activity is observed in absence of the salts. The inactivation at low-salt concentrations is reversible by increasing the salt concentration | Halorubrum xinjiangense | |
NaCl | maximum amylase activity at 4 M NaCl or 4.5 M KC, 70°C, and pH 8.5 | Halorubrum xinjiangense | |
NaCl | maximum amylase activity at 4 M NaCl or 4.5 M KCl, and retains about 6090% of the optimal activity at 23.5 M. The enzyme is less active in the presence of 0.51.5 M of each salt, and no activity is observed in absence of the salts. The inactivation at low-salt concentrations is reversible by increasing the salt concentration | Halorubrum xinjiangense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Halorubrum xinjiangense |
60000 | - |
1 * 60000, SDS-PAGE | Halorubrum xinjiangense |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 2 h, 50% v/v, 9.5% residual activity | Halorubrum xinjiangense |
Acetone | 20% (v/v), 25°C, pH 8.5, 2 h, 69% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 9.5% residual activity | Halorubrum xinjiangense |
Butanol | 2 h, 50% v/v, 121.3% residual activity | Halorubrum xinjiangense |
Butanol | 20% (v/v), 25°C, pH 8.5, 2 h, 130% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 121.3% residual activity | Halorubrum xinjiangense |
chloroform | 2 h, 50% v/v, 73.4% residual activity | Halorubrum xinjiangense |
chloroform | 20% (v/v), 25°C, pH 8.5, 2 h, 88.4% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 73.4% residual activity | Halorubrum xinjiangense |
cyclohexane | 2 h, 50% v/v, 124.5% residual activity | Halorubrum xinjiangense |
cyclohexane | 20% (v/v), 25°C, pH 8.5, 2 h, 178% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 124.5% residual activity | Halorubrum xinjiangense |
cyclohexanol | 2 h, 50% v/v, 58% residual activity | Halorubrum xinjiangense |
cyclohexanol | 20% (v/v), 25°C, pH 8.5, 2 h, 76% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 58% residual activity | Halorubrum xinjiangense |
cyclohexanone | 2 h, 50% v/v, 56% residual activity | Halorubrum xinjiangense |
cyclohexanone | 20% (v/v), 25°C, pH 8.5, 2 h, 64.5% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 56% residual activity | Halorubrum xinjiangense |
decane | 2 h, 50% v/v, 138% residual activity | Halorubrum xinjiangense |
decane | 20% (v/v), 25°C, pH 8.5, 2 h, 140.7% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 138% residual activity | Halorubrum xinjiangense |
dioxane | 2 h, 50% v/v, 90% residual activity | Halorubrum xinjiangense |
dioxane | 20% (v/v), 25°C, pH 8.5, 2 h, 100% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 90% residual activity | Halorubrum xinjiangense |
dodecane | 2 h, 50% v/v, 102% residual activity | Halorubrum xinjiangense |
dodecane | 20% (v/v), 25°C, pH 8.5, 2 h, 186% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 102% residual activity | Halorubrum xinjiangense |
Ethanol | 2 h, 50% v/v, 80% residual activity | Halorubrum xinjiangense |
Ethanol | 20% (v/v), 25°C, pH 8.5, 2 h, 110% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 80% residual activity | Halorubrum xinjiangense |
heptane | 2 h, 50% v/v, 91.7% residual activity | Halorubrum xinjiangense |
heptane | 20% (v/v), 25°C, pH 8.5, 2 h, 110% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 91.7% residual activity | Halorubrum xinjiangense |
hexadecane | 20% (v/v), 25°C, pH 8.5, 2 h, 260% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 145% residual activity | Halorubrum xinjiangense |
hexane | 2 h, 50% v/v, 101% residual activity | Halorubrum xinjiangense |
hexane | 20% (v/v), 25°C, pH 8.5, 2 h, 120% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 101% residual activity | Halorubrum xinjiangense |
Methanol | 2 h, 50% v/v, 49.3% residual activity | Halorubrum xinjiangense |
Methanol | 20% (v/v), 25°C, pH 8.5, 2 h, 75% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 49.3% residual activity | Halorubrum xinjiangense |
nonane | 2 h, 50% v/v, 136% residual activity | Halorubrum xinjiangense |
nonane | 20% (v/v), 25°C, pH 8.5, 2 h, 216.3% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 136% residual activity | Halorubrum xinjiangense |
octanol | 2 h, 50% v/v, 92.7% residual activity | Halorubrum xinjiangense |
octanol | 20% (v/v), 25°C, pH 8.5, 2 h, 185.3% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 92.7% residual activity | Halorubrum xinjiangense |
tetradecane | 2 h, 50% v/v, 126% residual activity | Halorubrum xinjiangense |
tetradecane | 20% (v/v), 25°C, pH 8.5, 2 h, 252% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 126% residual activity | Halorubrum xinjiangense |
toluene | 2 h, 50% v/v, 91.5% residual activity | Halorubrum xinjiangense |
toluene | 20% (v/v), 25°C, pH 8.5, 2 h, 146.6% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 91.5% residual activity | Halorubrum xinjiangense |
xylene | 2 h, 50% v/v, 141.4% residual activity | Halorubrum xinjiangense |
xylene | 20% (v/v), 25°C, pH 8.5, 2 h, 195% residual activity. 50% (v/v), 25°C, pH 8.5, 2 h, 141.4% residual activity | Halorubrum xinjiangense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halorubrum xinjiangense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Halorubrum xinjiangense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
487.5 | - |
pH 8.5, 70°C | Halorubrum xinjiangense |
487.5 | - |
pH 8.5, 70°C, reducing sugar produced | Halorubrum xinjiangense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ammylopectin + H2O | the enzyme exhibits higher activity toward soluble starch rather than amylose (55%), amylopectin (41%), dextrin (60%), and glycogen (29%) | Halorubrum xinjiangense | ? | - |
? | |
amylopektin + H2O | activity is 41% compared to the activity with soluble starch | Halorubrum xinjiangense | ? | - |
? | |
amylose + H2O | activity is 55% compared to the activity with soluble starch | Halorubrum xinjiangense | ? | - |
? | |
amylose + H2O | the enzyme exhibits higher activity toward soluble starch rather than amylose (55%), amylopectin (41%), dextrin (60%), and glycogen (29%) | Halorubrum xinjiangense | ? | - |
? | |
dextrin + H2O | activity is 60% compared to the activity with soluble starch | Halorubrum xinjiangense | ? | - |
? | |
dextrin + H2O | the enzyme exhibits higher activity toward soluble starch rather than amylose (55%), amylopectin (41%), dextrin (60%), and glycogen (29%) | Halorubrum xinjiangense | ? | - |
? | |
glycogen + H2O | activity is 29% compared to the activity with soluble starch | Halorubrum xinjiangense | ? | - |
? | |
glycogen + H2O | the enzyme exhibits higher activity toward soluble starch rather than amylose (55%), amylopectin (41%), dextrin (60%), and glycogen (29%) | Halorubrum xinjiangense | ? | - |
? | |
additional information | no hydrolytic activity is detected on alpha-cyclodextrin, beta-cyclodextrin, and pullulan | Halorubrum xinjiangense | ? | - |
? | |
additional information | no hydrolytic activity with alpha-cyclodextrin, beta-cyclodextrin or pullulan | Halorubrum xinjiangense | ? | - |
? | |
soluble starch + H2O | the enzyme hydrolyzes 69% of the wheat starch after a 2 h incubation at 70°C in an aqueous/hexadecane two-phase system. The main hydrolysis products are maltose, maltotriose, maltotetraose, maltopentaose, and maltohexaose showing that the amylase cleaves the internal alpha-1,4 linkages and indicating that the enzyme is an endo-type amylase. The enzyme exhibits higher activity toward soluble starch rather than amylose (55%), amylopectin (41%), dextrin (60%), and glycogen (29%) | Halorubrum xinjiangense | ? | - |
? | |
starch + H2O | the main hydrolysis products are maltose, maltotriose, maltotetraose, maltopentaose, and maltohexaose. The enzyme hydrolyzes 69% of the wheat starch after a 2 h incubation at 70°C in an aqueous/hexadecane two-phase system | Halorubrum xinjiangense | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Halorubrum xinjiangense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Halorubrum xinjiangense |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 85 | 55°C: about 45% of maximal activity, 85°C: about 40% of maximal activity | Halorubrum xinjiangense |
55 | 85 | 55°C: about 50% of maximal activity, 85°C: about 40% of maximal activity | Halorubrum xinjiangense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, 25% loss of activity | Halorubrum xinjiangense |
70 | - |
1 h, 31% loss of activity | Halorubrum xinjiangense |
75 | - |
30 min, 18% loss of activity | Halorubrum xinjiangense |
90 | - |
1 h, more than 60% loss of activity | Halorubrum xinjiangense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Halorubrum xinjiangense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | completely inactive in an acidic pH range of 3-5.5, pH 6.0: about 50% of maximal activity, pH 9.0: 68.3% of maximal activity, pH 11: 53.5% of maximal activity | Halorubrum xinjiangense |
6.5 | 11 | pH 6.5: about 50% of maximal activity, pH 11: about 55% of maximal activity | Halorubrum xinjiangense |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | loses its activity in acidic buffers (pH 36) | Halorubrum xinjiangense |
7 | - |
stable above | Halorubrum xinjiangense |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Halorubrum xinjiangense | - |
- |
4.5 |
Halorubrum xinjiangense | isoelectric focusing | - |
4.5 |