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Literature summary for 3.2.1.1 extracted from

  • Tachibana, Y.; Mendez Leclerre, M.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Cloning and expression of the alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus sp. KOD 1, and characterization of the enzyme (1996), J. Ferment. Bioeng., 82, 224-232.
No PubMed abstract available
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, T7 RNA polymerase expression system Pyrococcus sp.

General Stability

General Stability Organism
no loss of activity after dialysis of the purified enzyme overnight at 4°C against 100 mM MES buffer (pH 6.5) containing 1 mM EDTA Pyrococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
alpha-cyclodextrin 0.2%, 10% inhibition Pyrococcus sp.
beta-cyclodextrin 0.2%, 19% inhibition Pyrococcus sp.
CoCl2 1 mM, 52% inhibition Pyrococcus sp.
CuCl2 1 mM, complete inhibition Pyrococcus sp.
gamma-cyclodextrin 0.2%, 18% inhibition Pyrococcus sp.
glucosyl-alpha-cyclodextrin 0.2%, 19% inhibition Pyrococcus sp.
MnCl2 1 mM, 55% inhibition Pyrococcus sp.
SDS 1.0% 63% inhibition Pyrococcus sp.
ZnCl2 1 mM, complete inhibition Pyrococcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pyrococcus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Pyrococcus sp.
49456
-
 x * 49456, calculated from sequence Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp. O33476
-
-
Pyrococcus sp. KOD1 O33476
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Pyrococcus sp. ?
-
 ?
amylopectin + H2O
-
 Pyrococcus sp. KOD1 ?
-
 ?
amylose + H2O
-
 Pyrococcus sp. ?
-
 ?
amylose + H2O
-
 Pyrococcus sp. KOD1 ?
-
 ?
glycogen + H2O
-
 Pyrococcus sp. maltose + maltotriose major products ?
glycogen + H2O
-
 Pyrococcus sp. KOD1 maltose + maltotriose major products ?
additional information very weakly hydrolyzes pullulan to form panose or isopanose Pyrococcus sp. ?
-
 ?
additional information very weakly hydrolyzes pullulan to form panose or isopanose Pyrococcus sp. KOD1 ?
-
 ?
starch + H2O
-
 Pyrococcus sp. maltose + maltotriose major products ?
starch + H2O
-
 Pyrococcus sp. KOD1 maltose + maltotriose major products ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Pyrococcus sp.
? x * 49456, calculated from sequence Pyrococcus sp.

Synonyms

Synonyms Comment Organism
ApkA
-
 Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Pyrococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 100 60°C: about 60% of maximal activity, 100°C: about 80% of maximal activity Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 60 min, about 10% loss of activity, thermostability is enhanced in the presence of 2 mM Ca2+ Pyrococcus sp.
100
-
 60 min, in presence of 2 mM Ca2+, about 60% loss of activity Pyrococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2280
-
 amylose pH 6.5, 90°C Pyrococcus sp.
2300
-
 glycogen pH 6.5, 90°C Pyrococcus sp.
4180
-
 amylopectin pH 6.5, 90°C Pyrococcus sp.
4680
-
 starch pH 6.5, 90°C Pyrococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pyrococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 pH 4.5: about 40% of maximal activity, pH 8.0: about 40% of maximal activity Pyrococcus sp.