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Literature summary for 3.2.1.1 extracted from
- Alpha-amylase from germinating soybean (Glycine max) seeds - purification, characterization and sequential similarity of conserved and catalytic amino acid residues (2010), Phytochemistry, 71, 1657-1666.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | calcium stabilizes the conformation of alpha-amylase and also involved in substrate binding, but inhibits enzyme activity | Glycine max |  |
| Co2+ | - |
Glycine max | |
| EDTA | - |
Glycine max | |
| Mg2+ | - |
Glycine max | |
| Zn2+ | - |
Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | calcium stabilizes the conformation of alpha-amylase and also involved in substrate binding, but inhibits enzyme activity | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
gel filtration | Glycine max |
| 100000 | - |
1 * 100000, SDS-PAGE | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Glycine max | soybean alpha-amylase shows high specificity for its primary substrate starch, followed by amylopectin and dextrin (III), lower activity with amylopectin, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 400fold from germinating seeds to homogeneity by ammonium sulfate fractionation, heat treatment at 70°C for 10 min, beta-cyclodextrin affinity chromatography, and gel filtration | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 384.6 | - |
purified enzyme, pH 5.5, temperature not specified in the publication | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | soybean alpha-amylase shows high specificity for its primary substrate starch, followed by amylopectin and dextrin (III), lower activity with amylopectin, overview | Glycine max | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, SDS-PAGE | Glycine max |
| More | peptide mapping, overview | Glycine max |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the soybean alpha-amylase belongs to glycosyl hydrolase family 13 | Glycine max |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 85 | activity range | Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
thermal inactivation, first-order kinetics with rate constant equal to 0.0063/min | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Glycine max |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Glycine max | MALDITOF analysis | - |
4.85 |
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Release 2023.1 (January 2023)
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