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Literature summary for 3.2.1.1 extracted from
- A new protein of alpha-amylase activity from Lactococcus lactis (2010), J. Microbiol. Biotechnol., 20, 1307-1313.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene amy+, DNA and amino acid sequence determination and analysis, genome and phylogenetic analysis | Lactococcus lactis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Lactococcus lactis |  |
| Co2+ | - |
Lactococcus lactis | |
| Fe2+ | - |
Lactococcus lactis | |
| Hg2+ | - |
Lactococcus lactis | |
| Mg2+ | - |
Lactococcus lactis | |
| Mn2+ | - |
Lactococcus lactis | |
| Zn2+ | - |
Lactococcus lactis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Lactococcus lactis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Lactococcus lactis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect on enzyme activity by Ca2+, but structural stabilization of the protein molecule by calcium ions | Lactococcus lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119560 | - |
x * 119560, SDS-PAGE | Lactococcus lactis |
| 121000 | - |
gel filtration | Lactococcus lactis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | - |
a strain belonging to the amylolytic lactic acid bacteria, plasmid encoded gene amy+ | - |
| Lactococcus lactis IBB500 | - |
a strain belonging to the amylolytic lactic acid bacteria, plasmid encoded gene amy+ | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 14.4fold by two steps of ion exchanchromatography | Lactococcus lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2551 | - |
purified native enzyme, soluble starch, pH 4.5, 35°C | Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + H2O | - |
Lactococcus lactis | maltose + maltotriose | products of amylolytic attack are mixed oligosaccharides including mainly maltose and maltotriose | ? | |
| soluble starch + H2O | - |
Lactococcus lactis IBB500 | maltose + maltotriose | products of amylolytic attack are mixed oligosaccharides including mainly maltose and maltotriose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 119560, SDS-PAGE | Lactococcus lactis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Lactococcus lactis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | activity range | Lactococcus lactis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
purified enzyme, 60 min, stable | Lactococcus lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Lactococcus lactis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9 | activity range | Lactococcus lactis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5.5 | purified enzyme, stable | Lactococcus lactis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lactococcus lactis | - |
- |
7.4 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | hypothesis of horizontal gene transfer | Lactococcus lactis |
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