Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | effects of several commercial detergent products on the activities of the enzyme mutants, overview | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15 | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
M231L | construction of a thermostable variant BACDELTANC/DELTARS derived from the truncated alpha-amylase BACDELTANC, introduction of mutation M231L for enhancing the resistance towards chemical oxidation, and site-directed mutagenesis of the 483th codon in the gene to stop codon, resulting in the mutants BACDELTANC/DELTARS/M231L/DELTAC31. Mutants BACDELTANC/DELTARS/M231L and BACDELTANC/DELTARS/M231L/DELTAC31 are very similar to BACDELTANC in terms of specific activity, kinetic parameters, pH-activity profile, and the hydrolysis of raw starch, but the engineered enzymes show an increased half-life at 70°C | Bacillus sp. (in: Bacteria) |
General Stability | Organism |
---|---|
wild-type andmutant enzymes exhibit sensitivity towards GdnHCl-induced denaturation | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | wild-type and mutant enzymes exhibit sensitivity towards GdnHCl-induced denaturation, effects of several commercial detergent products on the activities of the enzyme mutants, overview | Bacillus sp. (in: Bacteria) | |
SDS | - |
Bacillus sp. (in: Bacteria) | |
Triton X-100 | - |
Bacillus sp. (in: Bacteria) | |
Tween-20 | - |
Bacillus sp. (in: Bacteria) | |
Tween-80 | - |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant mutant enzymes, overiew | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, recombinant wild-type enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) TS-23 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme mutants are more stable to H2O2 than the wild-type enzyme, overview | Bacillus sp. (in: Bacteria) |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
142.2 | - |
purified recombinant mutant BACDELTANC/DELTARS/M231L/DELTAC31, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) |
145.4 | - |
purified recombinant mutant BACDELTANC/DELTARS/M231L, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) |
158.9 | - |
purified recombinant mutant BACDELTANC/DELTARS, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) |
179.6 | - |
purified recombinant mutant BACDELTANC/M231L, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) |
193.3 | - |
purified recombinant mutant BACDELTANC, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
raw starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
raw starch + H2O | - |
Bacillus sp. (in: Bacteria) TS-23 | ? | - |
? | |
soluble starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
soluble starch + H2O | - |
Bacillus sp. (in: Bacteria) TS-23 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, recombinant wild-type enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at, substrate raw starch | Bacillus sp. (in: Bacteria) |
60 | - |
assay at, substrate soluble starch | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
139.1 | - |
soluble starch | mutant BACDELTANC/DELTARS/M231L/DELTAC31, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) | |
141.3 | - |
soluble starch | mutant BACDELTANC/DELTARS/M231L, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) | |
147.5 | - |
soluble starch | mutant BACDELTANC/M231L, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) | |
156.8 | - |
soluble starch | mutant BACDELTANC/DELTARS, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) | |
168.5 | - |
soluble starch | mutant BACDELTANC, pH 8.0, 60°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus sp. (in: Bacteria) |