Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1 Blue cells | Bacillus amyloliquefaciens |
Protein Variants | Comment | Organism |
---|---|---|
V289D | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
V289E | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
V289F | the mutant shows 48.9% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
V289G | the mutant shows 14.5% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
V289I | the mutant shows 20% more hydrolytic activity than the wild type enzyme | Bacillus amyloliquefaciens |
V289L | the mutant shows 36.4% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
V289P | the mutant shows 2.2% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
V289R | the mutation results in complete loss of the alpha-amylase activity | Bacillus amyloliquefaciens |
V289S | the mutant shows 5% activity compared to the wild type enzyme | Bacillus amyloliquefaciens |
V289Y | the mutant shows 9.2% activity compared to the wild type enzyme, the mutant has acquired a transglycosylation activity, which results in the change of product profile of the reaction, giving a longer oligosaccharide | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens MTCC 610 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltotetraose + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
maltotetraose + H2O | - |
Bacillus amyloliquefaciens MTCC 610 | ? | - |
? | |
starch + H2O | - |
Bacillus amyloliquefaciens | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Bacillus amyloliquefaciens MTCC 610 | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmyA | - |
Bacillus amyloliquefaciens |
endo-1,4-alpha-D-glucan glucohydrolase | - |
Bacillus amyloliquefaciens |