Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Sitophilus zeamais |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | additional provision of Cl- (up to 30 mM NaCl) in the reaction mixture does not affect significantly the activity of alpha-amylases | Sitophilus zeamais |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53700 | - |
SDS-PAGE | Sitophilus zeamais |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Sitophilus zeamais | the alpha-amylase from the resistant no-cost strain exhibits higher activity towards starch compared to the resistant cost strain | malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sitophilus zeamais | - |
maize weevil, insecticide-susceptible, resistant no-cost, and resistant cost strains | - |
Purification (Comment) | Organism |
---|---|
glycogen precipitation and DEAE-Sephacel gel filtration | Sitophilus zeamais |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | the alpha-amylase from the resistant no-cost strain exhibits higher activity towards starch compared to the resistant cost strain | Sitophilus zeamais | malto-oligosaccharides | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
24.49 | - |
alpha-amylase from pyrethroid-resistant cost Sitophilus zeamais strain, at 37°C | Sitophilus zeamais | acarbose | |
41 | - |
alpha-amylase from pyrethroid-susceptible Sitophilus zeamais strain, at 37°C | Sitophilus zeamais | acarbose | |
63.8 | - |
alpha-amylase from pyrethroid-resistant no-cost Sitophilus zeamais strain, at 37°C | Sitophilus zeamais | acarbose |