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Literature summary for 3.2.1.1 extracted from

  • Lopes, K.V.; Silva, L.B.; Reis, A.P.; Oliveira, M.G.; Guedes, R.N.
    Modified alpha-amylase activity among insecticide-resistant and -susceptible strains of the maize weevil, Sitophilus zeamais (2010), J. Insect Physiol., 56, 1050-1057.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
Sitophilus zeamais

Metals/Ions

Metals/Ions Comment Organism Structure
additional information additional provision of Cl- (up to 30 mM NaCl) in the reaction mixture does not affect significantly the activity of alpha-amylases Sitophilus zeamais

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53700
-
SDS-PAGE Sitophilus zeamais

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Sitophilus zeamais the alpha-amylase from the resistant no-cost strain exhibits higher activity towards starch compared to the resistant cost strain malto-oligosaccharides
-
?

Organism

Organism UniProt Comment Textmining
Sitophilus zeamais
-
maize weevil, insecticide-susceptible, resistant no-cost, and resistant cost strains
-

Purification (Commentary)

Purification (Comment) Organism
glycogen precipitation and DEAE-Sephacel gel filtration Sitophilus zeamais

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O the alpha-amylase from the resistant no-cost strain exhibits higher activity towards starch compared to the resistant cost strain Sitophilus zeamais malto-oligosaccharides
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
24.49
-
alpha-amylase from pyrethroid-resistant cost Sitophilus zeamais strain, at 37°C Sitophilus zeamais acarbose
41
-
alpha-amylase from pyrethroid-susceptible Sitophilus zeamais strain, at 37°C Sitophilus zeamais acarbose
63.8
-
alpha-amylase from pyrethroid-resistant no-cost Sitophilus zeamais strain, at 37°C Sitophilus zeamais acarbose