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Literature summary for 3.2.1.1 extracted from

  • Dheeran, P.; Kumar, S.; Jaiswal, Y.K.; Adhikari, D.K.
    Characterization of hyperthermostable alpha-amylase from Geobacillus sp. IIPTN (2010), Appl. Microbiol. Biotechnol., 86, 1857-1866.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SDS SDS enhances the activity 42% more than the original activity at 5 mM Geobacillus sp.
Urea urea enhances the activity 47% more than the original activity at 5 mM Geobacillus sp.

General Stability

General Stability Organism
the enzyme has resistance against protease Geobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
EDTA 10% residual activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Hg2+ complete inhibition at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Mg2+ 67% residual activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Zn2+ 50% residual activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information apparent Km is 36 mg/ml using cassava starch as substrate Geobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 163% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Ca2+ 180% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Co2+ 162% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Fe3+ 133% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
K+ 131% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Mn2+ 191% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.
Na+ 142% relative activity at 5 mM, at 80°C and pH 5.0 Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
SDS-PAGE Geobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Geobacillus sp. 86% activity compared to potato soluble starch ?
-
?
amylopectin + H2O Geobacillus sp. IIPTN / MTCC 5319 86% activity compared to potato soluble starch ?
-
?
cassava starch + H2O Geobacillus sp. 129% activity compared to potato soluble starch ?
-
?
cassava starch + H2O Geobacillus sp. IIPTN / MTCC 5319 129% activity compared to potato soluble starch ?
-
?
corn flour + H2O Geobacillus sp. 58% activity compared to potato soluble starch ?
-
?
corn flour + H2O Geobacillus sp. IIPTN / MTCC 5319 58% activity compared to potato soluble starch ?
-
?
corn starch + H2O Geobacillus sp. 107% activity compared to potato soluble starch ?
-
?
corn starch + H2O Geobacillus sp. IIPTN / MTCC 5319 107% activity compared to potato soluble starch ?
-
?
potato soluble starch + H2O Geobacillus sp. 100% activity ?
-
?
potato soluble starch + H2O Geobacillus sp. IIPTN / MTCC 5319 100% activity ?
-
?
sweet sorghum starch + H2O Geobacillus sp. 62% activity compared to potato soluble starch ?
-
?
tapioca root starch + H2O Geobacillus sp. 88% activity compared to potato soluble starch ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
-
-
Geobacillus sp. IIPTN / MTCC 5319
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, hydroxyapatite column chromatography, and Macro Prep High S support column chromatography Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0145
-
crude extract, at 80°C Geobacillus sp.
1.2
-
after 82fold purification, at 80°C Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 86% activity compared to potato soluble starch Geobacillus sp. ?
-
?
amylopectin + H2O 86% activity compared to potato soluble starch Geobacillus sp. IIPTN / MTCC 5319 ?
-
?
cassava starch + H2O 129% activity compared to potato soluble starch Geobacillus sp. ?
-
?
cassava starch + H2O 129% activity compared to potato soluble starch Geobacillus sp. IIPTN / MTCC 5319 ?
-
?
corn flour + H2O 58% activity compared to potato soluble starch Geobacillus sp. ?
-
?
corn flour + H2O 58% activity compared to potato soluble starch Geobacillus sp. IIPTN / MTCC 5319 ?
-
?
corn starch + H2O 107% activity compared to potato soluble starch Geobacillus sp. ?
-
?
corn starch + H2O 107% activity compared to potato soluble starch Geobacillus sp. IIPTN / MTCC 5319 ?
-
?
potato soluble starch + H2O 100% activity Geobacillus sp. ?
-
?
potato soluble starch + H2O 100% activity Geobacillus sp. IIPTN / MTCC 5319 ?
-
?
sweet sorghum starch + H2O 62% activity compared to potato soluble starch Geobacillus sp. ?
-
?
tapioca root starch + H2O 88% activity compared to potato soluble starch Geobacillus sp. ?
-
?

Synonyms

Synonyms Comment Organism
1,4-alpha-D-glucan glucanohydrolase and endoamylase
-
Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 120 the activity increases sharply from 40°C with gradual increase in temperature up to 80°C and it declines gradually with further rise in temperature, the enzyme is 75% active at 120°C Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Geobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 19% and 36% of maximum activity at pH 4.5 and 9.5, respectively Geobacillus sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
at 80°C and pH 5.0 Geobacillus sp. Zn2+

Expression

Organism Comment Expression
Geobacillus sp. the maximum production of enzyme is observed on the medium containing 0.5% (w/v) cassava starch, 0.5% (w/v) tryptone, yeast extract, soymeal, ammonium chloride, and ammonium nitrate enhance the amylase production up