Literature summary for 3.2.1.1 extracted from

Tripathi, P.; Hofmann, H.; Kayastha, A.M.; Ulbrich-Hofmann, R.
Conformational stability and integrity of alpha-amylase from mung beans: evidence of kinetic intermediate in GdmCl-induced unfolding (2008), Biophys. Chem., 137, 95-99.

Crystallization (Commentary)

Crystallization (Comment)	Organism
the circular dichroism spectrum shows two minima at 208 and 222 nm. Secondary structure analysis of the spectra estimated 31% alpha-helix, 15% beta-sheet, and 54% random coils	Vigna radiata

General Stability

General Stability	Organism
unfolding kinetics with guanidinium hydrochloride followed at concentrations in the range of 2.8-7.8 M by kinetic stopped-flow fluorescence measurements. At concentrations higher than 6 M, two unfolding phases are detected, whereas one phase is observed at concentrations lower than 5 M. These results suggest that the first reversible step of unfolding is slower than the second irreversible step at low guanidinium hydrochloride concentrations, whereas the rates of these two steps are opposite at high concentrations	Vigna radiata

Organism

unfolding kinetics with guanidinium hydrochloride followed at concentrations in the range of 2.8-7.8 M by kinetic stopped-flow fluorescence measurements. At concentrations higher than 6 M, two unfolding phases are detected, whereas one phase is observed at concentrations lower than 5 M. These results suggest that the first reversible step of unfolding is slower than the second irreversible step at low guanidinium hydrochloride concentrations, whereas the rates of these two steps are opposite at high concentrations

Vigna radiata

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	a distinct decrease of enzyme activity. The activity can not be regained after the addition of excess CaCl2, demonstrating the irreversibility of inactivation	Vigna radiata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CaCl2	incubated in different concentrations 0.5-100 mM at 25°C overnight, with 10 mM EDTA, significant activation, with two apparent dissociation constants K1: 0.3 mM and K2: 5.4 mM	Vigna radiata

Organism

Organism	UniProt	Comment	Textmining
Vigna radiata	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
from seeds	Vigna radiata

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Vigna radiata	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
starch + H2O	pH 5.5, 55°C	Vigna radiata	?	-	?

Synonyms

Synonyms	Comment	Organism
VrAmy	-	Vigna radiata

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	assay at	Vigna radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
48	-	transition temperature T1/2 in the presence of 10 mM EDTA, thermal unfolding observed by circular dichroism spectrum measurements	Vigna radiata
87	-	transition temperature T1/2 in the presence of 5 mM CaCl2, thermal unfolding observed by circular dichroism spectrum measurements	Vigna radiata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	assay at	Vigna radiata