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Literature summary for 3.2.1.1 extracted from

  • van der Kaaij, R.M.; Janecek, S.; van der Maarel, M.J.; Dijkhuizen, L.
    Phylogenetic and biochemical characterization of a novel cluster of intracellular fungal alpha-amylase enzymes (2007), Microbiology, 153, 4003-4015.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
AmyD Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for potato starch in the hydrolysis reaction is between 0.02 and 0.05% starch (w/v), AmyD Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular AmyD Aspergillus niger 5622
-

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
AmyD Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
 AmyD Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 66% of the activity with potato starch, AmyD Aspergillus niger ?
-
 ?
amylose + H2O 101% of the activity with potato starch, AmyD Aspergillus niger ?
-
 ?
glycogen + H2O 3.6% of the activity with potato starch, AmyD Aspergillus niger ?
-
 ?
starch + H2O AmyD produces mainly maltotriose Aspergillus niger maltotriose + ?
-
 ?

Synonyms

Synonyms Comment Organism
AmyD
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 10 min, stable Aspergillus niger
40
-
 10 min, about 15% loss of activity Aspergillus niger
45
-
 10 min, about 80% loss of activity Aspergillus niger
50
-
 10 min, complete loss of activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 AmyD Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity, AmyD Aspergillus niger