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Literature summary for 3.2.1.1 extracted from

  • Babacan, S.; Rand, A.G.
    Characterization of honey amylase (2007), J. Food Sci., 72, C050-C055.
    View publication on PubMed

Application

Application Comment Organism
food industry starch has a protective effect on thermal stability of honey amylase. Therefore, it might be critical to process or control the amylase in honey before incorporation into starch-containing foods to aid in the preservation of starch functionality Apis mellifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM-value for soluble starch is 0.72mg/ml Apis mellifera

Metals/Ions

Metals/Ions Comment Organism Structure
CuCl 1 mM, 34% inhibition Apis mellifera
HgCl2 1 mM, 13% inhibition Apis mellifera
MgCl2 1 mM, 22% inhibition Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
honey
-
Apis mellifera
-

Storage Stability

Storage Stability Organism
4°C, Tris-chloride buffer, pH 7.4, stable for 7 weeks Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
Apis mellifera ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Apis mellifera

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 25°C: about 40% of maximal activity, 70°C: about 70% of maximal activity Apis mellifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 7.4, half-life: 23.4 min Apis mellifera
50
-
pH 7.4, half-life: 6.16 min Apis mellifera
63
-
pH 7.4, complete inactivation within 8 min, half-life: 165 min. The soluble starch increases the half-life of the purified amylase from 1.65 min to 8.98 min at 63°C Apis mellifera
71
-
pH 7.4, complete inactivation within 2 min, half-life: 24 s Apis mellifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6 5.3 at 40°C Apis mellifera

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 55% of maximal activity, pH 6: about 50% of maximal activity Apis mellifera

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
purified honey amylase is less pH-stable than in its natural environment Apis mellifera
3.6
-
1 h, complete loss of activity Apis mellifera
3.8
-
1 h, 90% loss of activity Apis mellifera
4
-
1 h, 78% loss of activity Apis mellifera
7
-
1 h, optimum stability Apis mellifera
8
-
1 h, 10% loss of activity Apis mellifera
8.5
-
1 h, 29% loss of activity Apis mellifera
9
-
1 h, 31% loss of activity Apis mellifera