Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, alpha-amylase I | Halalkalibacterium halodurans |
expression in Escherichia coli, alpha-amylase II | Halalkalibacterium halodurans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, 40°C, 30 min, 12% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
BaCl2 | 1 mM, 40°C, 30 min, 16% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
CoCl2 | 1 mM, 40°C, 30 min, 22% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
CuSO4 | 1 mM, 40°C, 30 min, 25% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
EDTA | 1 mM, 40°C, 30 min, complete loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
FeCl3 | 1 mM, 40°C, 30 min, 21% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
FeSO4 | 1 mM, 40°C, 30 min, 86% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
HgCl2 | 1 mM, 40°C, 30 min, 95% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
MnCl2 | 1 mM, 40°C, 30 min, 46% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
NiSO4 | 1 mM, 40°C, 30 min, 79% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
PbCl2 | 1 mM, 40°C, 30 min, 35% loss of activity, alpha-amylase I | Halalkalibacterium halodurans | |
ZnSO4 | 1 mM, 40°C, 30 min, 47% loss of activity, alpha-amylase I | Halalkalibacterium halodurans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
alpha-amylase I, non-denaturing PAGE | Halalkalibacterium halodurans |
75000 | - |
1 * 75000, alpha-amylase II, SDS-PAGE | Halalkalibacterium halodurans |
87000 | - |
alpha-amylase II, non-denaturing PAGE | Halalkalibacterium halodurans |
100000 | - |
1 * 100000, alpha-amylase I, SDS-PAGE | Halalkalibacterium halodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
- |
- |
Halalkalibacterium halodurans 38C-2-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
alpha-amylase I | Halalkalibacterium halodurans |
alpha-amylase II | Halalkalibacterium halodurans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
alpha-amylase II | Halalkalibacterium halodurans |
130 | - |
alpha-amylase I | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 121% of the activity with soluble starch | Halalkalibacterium halodurans | ? | - |
? | |
amylopectin + H2O | 121% of the activity with soluble starch | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
amylose + H2O | 30% of the activity with soluble starch | Halalkalibacterium halodurans | ? | - |
? | |
amylose + H2O | 30% of the activity with soluble starch | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
glycogen + H2O | 104% of the activity with soluble starch | Halalkalibacterium halodurans | ? | - |
? | |
glycogen + H2O | 104% of the activity with soluble starch | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
additional information | no hydrolytic activity towards pullulan or dextran | Halalkalibacterium halodurans | ? | - |
? | |
additional information | no hydrolytic activity towards pullulan or dextran | Halalkalibacterium halodurans 38C-2-1 | ? | - |
? | |
soluble starch + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
soluble starch + H2O | - |
Halalkalibacterium halodurans 38C-2-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, alpha-amylase I, SDS-PAGE | Halalkalibacterium halodurans |
monomer | 1 * 75000, alpha-amylase II, SDS-PAGE | Halalkalibacterium halodurans |
Synonyms | Comment | Organism |
---|---|---|
alpha-amylase I | - |
Halalkalibacterium halodurans |
alpha-amylase II | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
alpha-amylase II | Halalkalibacterium halodurans |
50 | 60 | alpha-amylase I | Halalkalibacterium halodurans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: 38% of maximal activity, 50-60: maximal activity, alpha-amylase II | Halalkalibacterium halodurans |
30 | 60 | 30°C: 42% of maximal activity, 50-60°C: maximal activity, alpha-amylase I | Halalkalibacterium halodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, 58% loss of activity, alpha-amylase II | Halalkalibacterium halodurans |
50 | - |
30 min, less than 5% loss of activity, alpha-amylase I | Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
alpha-amylase I | Halalkalibacterium halodurans |
10 | 11 | alpha-amylase I | Halalkalibacterium halodurans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | 4°C, 24 h, stable, alpha-amylase I | Halalkalibacterium halodurans |