Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 10 mM, 58% inhibition | Hyphopichia burtonii | |
Cd2+ | 10 mM, complete inhibition | Hyphopichia burtonii | |
CH2ICOOH | 10 mM, complete inhibition | Hyphopichia burtonii | |
Cu2+ | 10 mM, 58% inhibition | Hyphopichia burtonii | |
DTNB | 10 mM, complete inhibition | Hyphopichia burtonii | |
EDTA | 10 mM, 57% inhibition | Hyphopichia burtonii | |
Hg2+ | 10 mM, 55% inhibition | Hyphopichia burtonii | |
K+ | 10 mM, 23% inhibition | Hyphopichia burtonii | |
Mg2+ | 10 mM, 13% inhibition | Hyphopichia burtonii | |
Ni2+ | 10 mM, 42% inhibition | Hyphopichia burtonii | |
Pb2+ | 10 mM, 45% inhibition | Hyphopichia burtonii | |
Zn2+ | 10 mM, 60% inhibition | Hyphopichia burtonii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Hyphopichia burtonii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 1.35fold activation | Hyphopichia burtonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, SDS-PAGE | Hyphopichia burtonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphopichia burtonii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Hyphopichia burtonii |
Purification (Comment) | Organism |
---|---|
- |
Hyphopichia burtonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 95% of the activity with soluble starch | Hyphopichia burtonii | ? | - |
? | |
amylose + H2O | 137% of the activity with soluble starch | Hyphopichia burtonii | ? | - |
? | |
gamma-cyclodextrin + H2O | 21% of the activity with soluble starch | Hyphopichia burtonii | ? | - |
? | |
isomaltose + H2O | 23% of the activity with soluble starch | Hyphopichia burtonii | ? | - |
? | |
maltohexaose + H2O | 58% of the activity with soluble starch | Hyphopichia burtonii | ? | - |
? | |
soluble starch + H2O | - |
Hyphopichia burtonii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, SDS-PAGE | Hyphopichia burtonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Hyphopichia burtonii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | 20°C: about 70% of maximal activity, 55°C: about 70% of maximal activity | Hyphopichia burtonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.0, 30 min, about 20% loss of activity | Hyphopichia burtonii |
55 | - |
pH 5.0, 30 min, about 60% loss of activity | Hyphopichia burtonii |
60 | - |
pH 5.0, 30 min, complete loss of activity | Hyphopichia burtonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Hyphopichia burtonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | pH 3.5: about 40% of maximal activity, pH 7.0: about 45% of maximal activity | Hyphopichia burtonii |
4 | 5.5 | the enzyme shows activity higher than 80% in the range pH 4.0 to 5.5 | Hyphopichia burtonii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
24 h, complete loss of activity | Hyphopichia burtonii |
4 | 4.5 | 24 h, no loss of activity | Hyphopichia burtonii |
5 | - |
24 h, about 10% loss of activity | Hyphopichia burtonii |
6 | - |
24 h, about 20% loss of activity | Hyphopichia burtonii |
7 | - |
24 h, about 50% loss of activity | Hyphopichia burtonii |
8 | - |
24 h, about 80% loss of activity | Hyphopichia burtonii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hyphopichia burtonii | chromatofocusing | - |
4.8 |