Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Br- | greater affinity for the enzyme than chloride and a comparable relative activity | Homo sapiens | |
Cl- | allosteric activation. The enzyme is essentially inactive in the absence of chloride ion | Homo sapiens | |
ClO3- | activates, optimal concentration is 0.2 mM | Homo sapiens | |
HCOO- | activates, optimal concentration is 1 mM | Homo sapiens | |
I- | lower affinity for the enzyme than chloride and higher relative activity | Homo sapiens | |
N3- | activates, optimal concentration is 1 mM | Homo sapiens | |
NO2- | activates, optimal concentration is 1 mM | Homo sapiens | |
NO3- | activates, optimal concentration is 0.1 mM | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
recombinant wild-type and N298S variant HPAs are crystallized using the hanging drop vapor diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N298S | variant of the enzyme has an approximate 200fold reduction in affinity for chloride ion | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.1 mM NO3- | Homo sapiens | |
0.57 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 1 mM N3- | Homo sapiens | |
0.58 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.2 mM ClO3- | Homo sapiens | |
0.58 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 1 mM NO3- | Homo sapiens | |
0.69 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 1 mM N3- | Homo sapiens | |
0.76 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.01 mM Cl- | Homo sapiens | |
1.2 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.01 mM Br- | Homo sapiens | |
1.3 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.01 mM I- | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04746 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-4-nitrophenyl alpha-maltotrioside + H2O | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.01 mM Br- | Homo sapiens | |
2 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.2 mM ClO3- | Homo sapiens | |
2.1 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.01 mM I- | Homo sapiens | |
2.2 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.1 mM NO3- | Homo sapiens | |
2.4 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 0.01 mM Cl- | Homo sapiens | |
3.5 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 1 mM NO2- | Homo sapiens | |
4.4 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 1 mM N3- | Homo sapiens | |
15.8 | - |
2-chloro-4-nitrophenyl alpha-maltotrioside | in presence of 1 mM N3- | Homo sapiens |