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Literature summary for 3.2.1.1 extracted from

  • Lyhne-Iversen, L.; Hobley, T.J.; Kaasgaard, S.G.; Harris, P.
    Structure of Bacillus halmapalus alpha-amylase crystallized with and without the substrate analogue acarbose and maltose (2006), Acta Crystallogr. Sect. F, 62, 849-854.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method. The first crystal form is obtained by crystallization of BHA at room temperature in the presence of acarbose and maltose, resolution of 1.9 A. The crystal belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 47.0, b = 73.5, c = 151.1 A. The second crystal form is obtained by pH-induced crystallization of BHA in a MES-HEPES-boric acid buffer (MHB buffer) at 30°C. The solubility of BHA in MHB has a retrograde temperature dependency and crystallization of BHA is only possible by raising the temperature to at least 25°C. Data are collected at cryogenic temperature to a resolution of 2.0 A. The crystal belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.6, b = 59.0, c = 209.8 A Sutcliffiella halmapala

Organism

Organism UniProt Comment Textmining
Sutcliffiella halmapala
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Synonyms

Synonyms Comment Organism
BHA
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Sutcliffiella halmapala