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Literature summary for 3.2.1.1 extracted from

  • Patel, A.K.; Nampoothiri, K.M.; Ramachandran, S.; Szakacs, G.; Pandey, A.
    Partial purification and characterization of alpha-amylase produced by Aspergillus oryzae using spent-brewing grains (2005), Indian J. Biotechnol., 4, 336-341.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Aspergillus oryzae
Hg2+
-
Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizes the partially purified enzyme against thermal inactivation Aspergillus oryzae
Fe2+ activates Aspergillus oryzae
Mn2+ activates Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
x * 66000, SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
IFO 30103
-

Purification (Commentary)

Purification (Comment) Organism
partial 2.4fold purification of native enzyme by ammonium sulfate fractionation Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
additional information soli fermentation on spent-brewing grains Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme activity in solid fermentation cultures grown on spent-brewing grains Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Aspergillus oryzae ?
-
?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
partially purified enzyme, rapid loss of activity, Ca2+ protects Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus oryzae