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Literature summary for 3.2.1.1 extracted from
- Comparative characterization of raw starch hydrolyzing a-amylases from various Bacillus strains (2005), Enzyme Microb. Technol., 37, 410-416.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE | Bacillus subtilis |
| 47000 | - |
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE | Bacillus subtilis |
| 64000 | - |
x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Bacillus subtilis | - |
malto-oligosaccharides | - |
? |  |
| starch + H2O | Bacillus cereus | - |
malto-oligosaccharides | - |
? | |
| starch + H2O | Bacillus licheniformis | - |
malto-oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
strains IFO 3032 and IFO 3034 | - |
| Bacillus licheniformis | - |
diverse strains, overview | - |
| Bacillus subtilis | - |
diverse strains, overview, isozymes RBLA, RBSA-1 and BSA-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| separation of two native isozymes RBSA-1 and BSA-2 from strain IFO 3108 by gel filtration | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
adsorption to and hydrolysis of raw starch from corn and potato, overview | Bacillus subtilis |
| additional information | - |
adsorption to and hydrolysis of raw starch from corn and potato, overview | Bacillus cereus |
| additional information | - |
adsorption to and hydrolysis of raw starch from corn and potato, overview | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gelatinized starch + H2O | isozyme RBLA prefers gelatinized starch | Bacillus subtilis | maltotetraose + maltopentaose + maltohexaose | - |
? | |
| additional information | adsorption to and hydrolysis of raw starch from corn and potato, effect on isozymes, overview, isozyme RBLA is a liquifying alpha-amylase | Bacillus subtilis | ? | - |
? | |
| additional information | adsorption to and hydrolysis of raw starch from corn and potato, overview | Bacillus cereus | ? | - |
? | |
| additional information | adsorption to and hydrolysis of raw starch from corn and potato, overview | Bacillus licheniformis | ? | - |
? | |
| raw starch + H2O | isozymes RBSA-1 and BSA-2, raw starch from corn and potato, the latter is preferred | Bacillus subtilis | malto-oligosaccharides | - |
? | |
| starch + H2O | - |
Bacillus subtilis | malto-oligosaccharides | - |
? | |
| starch + H2O | - |
Bacillus cereus | malto-oligosaccharides | - |
? | |
| starch + H2O | - |
Bacillus licheniformis | malto-oligosaccharides | - |
? | |
| starch + H2O | raw starch from corn and potato | Bacillus cereus | malto-oligosaccharides | - |
? | |
| starch + H2O | raw starch from corn and potato, the latter is preferred | Bacillus licheniformis | malto-oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, isozyme RBSA-1, SDS-PAGE, x * 47000, isozyme BSA-2, SDS-PAGE, x * 46000, isozyme RBLA, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BSA-2 | - |
Bacillus subtilis |
| RBLA | - |
Bacillus subtilis |
| RBSA-1 | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Bacillus cereus |
| 50 | - |
assay at | Bacillus licheniformis |
| 65 | - |
isozymes RBSA-1 and BSA-2 | Bacillus subtilis |
| 65 | 70 | isozyme RBLA | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, isozymes RBSA-1 and BSA-2, stable up to | Bacillus subtilis |
| 60 | - |
10 min, isozyme RBLA, stable up to | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Bacillus cereus |
| 6 | - |
assay at | Bacillus licheniformis |
| 6 | - |
isozymes RBLA, RBSA-1 and BSA-2 | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | isozyme RBLA, stable for 24 h at 4°C | Bacillus subtilis |
| 5.5 | 9 | isozymes RBSA-1 and BSA-2, stable for 24 h at 4°C | Bacillus subtilis |
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