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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of alpha-amylase from the infective juveniles of the nematode Heterorhabditis bacteriophora (2004), Comp. Biochem. Physiol. B, 139, 1-9.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 98% inhibition of isozyme alpha-amylase I at 0.5 mM | Heterorhabditis bacteriophora |  |
| CoCl2 | 21% inhibition of isozyme alpha-amylase I at 0.5 mM, 29% at 5 mM | Heterorhabditis bacteriophora | |
| EDTA | 92% inhibition of isozyme alpha-amylase I at 1 mM | Heterorhabditis bacteriophora | |
| iodoacetic acid | 88% inhibition of isozyme alpha-amylase I at 0.5 mM | Heterorhabditis bacteriophora | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.5 | - |
starch | pH 7.0, 40°C, alpha-amylase I | Heterorhabditis bacteriophora | |
| 9.6 | - |
glycogen | pH 7.0, 40 °C, alpha-amylase I | Heterorhabditis bacteriophora | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 76% activation of isozyme alpha-amylase I at 1 mM | Heterorhabditis bacteriophora | |
| additional information | alpha-amylase I is no affected by NaNO3, BaCl2, and MgCl2 | Heterorhabditis bacteriophora | |
| NaCl | 18% activation of isozyme alpha-amylase I at 1 mM, 21% at 10 mM | Heterorhabditis bacteriophora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
1 * 46000, alpha-amylase I, SDS-PAGE | Heterorhabditis bacteriophora |
| 47000 | - |
alpha-amylase I, gel filtration | Heterorhabditis bacteriophora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
| amylose + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
| glycogen + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
| starch + H2O | Heterorhabditis bacteriophora | - |
malto-oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heterorhabditis bacteriophora | - |
entomopathogenic parasitic nematode, strain EGG, three isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| separation of three native isozymes from the infective juvenile of the nematode, purification of isozyme alpha-amylase I 74fold to homogeneity, by two steps of ion exchange chromatography and gel filtration | Heterorhabditis bacteriophora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | infective juvenile of the nematode, three isozymes, alpha-amylase I is the major isozyme | Heterorhabditis bacteriophora | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 98.4 | - |
purified alpha-amylase I | Heterorhabditis bacteriophora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
| amylose + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
| amylose + H2O | preferred substrate | Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
| glycogen + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
| glycogen + H2O | substrate from oyster | Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
| starch + H2O | - |
Heterorhabditis bacteriophora | malto-oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46000, alpha-amylase I, SDS-PAGE | Heterorhabditis bacteriophora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-amylase I | - |
Heterorhabditis bacteriophora |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isozyme alpha-amylase I | Heterorhabditis bacteriophora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified alpha-amylase I, stable up to, unstable above | Heterorhabditis bacteriophora |
| 50 | - |
30 min, loss of 62% activity of purified alpha-amylase I | Heterorhabditis bacteriophora |
| 60 | - |
30 min, loss of 78% activity of purified alpha-amylase I | Heterorhabditis bacteriophora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
isozyme alpha-amylase I | Heterorhabditis bacteriophora |
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