Cloned (Comment) | Organism |
---|---|
PTFA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) and MC1061 | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pyrococcus furiosus | |
0.52 | - |
starch | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
2.16 | - |
beta-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
2.61 | - |
alpha-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
5.12 | - |
gamma-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
11.1 | - |
acarbose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
62.9 | - |
maltotriose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76084 | - |
x * 76084, amino acid sequence calculation | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
bifunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant PFTA 32.6fold from Escherichia coli | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pyrococcus furiosus |
176.4 | - |
purified recombinant enzyme, substrate beta-cyclodextrin | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-maltopentaoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + alpha-D-maltopentaoside | - |
? | |
acarbose + H2O | the substrate is a potent inhibitor of alpha-amylases, cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
alpha-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
beta-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | panose + maltoheptaose | - |
? | |
gamma-cyclodextrin + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | ? | - |
? | |
maltooligosaccharides + H2O | alpha-amylase activity | Pyrococcus furiosus | maltotriose + maltotetraose | main products | ? | |
maltotriose + H2O | very low activity | Pyrococcus furiosus | ? | - |
? | |
additional information | substrate specificity, hydrolytic pattern, PFTA is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity, but no transglycosylation activity, overview | Pyrococcus furiosus | ? | - |
? | |
pullulan + H2O | cyclomaltodextrinase activity | Pyrococcus furiosus | panose + maltoheptaose | - |
? | |
starch + H2O | alpha-amylase activity, preferred substrate | Pyrococcus furiosus | maltotriose + maltotetraose | main products | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76084, amino acid sequence calculation | Pyrococcus furiosus |
More | primary structure scheme | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.2.1.54, cyclomaltodextrinase, the enzyme belongs to glycoside hydrolase family 13 | Pyrococcus furiosus |
PFTA | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
recombinant enzyme | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
highly thermostable recombinant enzyme | Pyrococcus furiosus |
85 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, completely stable for 120 min | Pyrococcus furiosus |
90 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 15-20% activity within 120 min | Pyrococcus furiosus |
95 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 25% activity within 120 min | Pyrococcus furiosus |
100 | - |
purified recombinant enzyme, sodium acetate buffer, pH 5.0, loss of 65% activity within 60 min | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
starch | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
173 | - |
gamma-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
196 | - |
beta-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
228 | - |
acarbose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
241 | - |
alpha-cyclodextrin | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus | |
268 | - |
maltotriose | pH 4.5, 90°C, recombinant enzyme | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Pyrococcus furiosus |