Literature summary for 3.2.1.1 extracted from

Suvd, D.; Fujimoto, Z.; Takase, K.; Matsumura, M.; Mizuno, H.
Crystal structure of Bacillus stearothermophilus alpha-amylase: possible factors determining the thermostability (2001), J. Biochem., 129, 461-468.

Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Bacillus subtilis	Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
tiny needle crystals are formed in 35 mM Na-acetate, pH 4.6, 35 mM CaCl2, 6.25% 2-propanol in the presence of 1.23% of the pseudo-oligosaccharide inhibitor acarbose at a protein concentration of 10 mg/ml, crystals diffract to 2.0 A resolution	Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha-1,4-glucan + H2O	Geobacillus stearothermophilus	-	fragments of alpha-1,4-glucan	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant alpha-amylase	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-1,4-glucan + H2O	-	Geobacillus stearothermophilus	fragments of alpha-1,4-glucan	-	?

Synonyms

Synonyms	Comment	Organism
BSTA	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	50% loss of activity after 50 min at pH 6.5	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Geobacillus stearothermophilus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Geobacillus stearothermophilus	-	-	6.9

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Release 2023.1 (January 2023)