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Literature summary for 3.2.1.1 extracted from

  • Petrova, S.D.; Ilieva, S.Z.; Bakalova, N.G.; Atev, A.P.; Bhat, M.K.; Kolev, D.N.
    Production and characterization of extracellular alpha-amylases from the thermophilic fungus Thermomyces lanuginosus (wild and mutant strains) (2000), Biotechnol. Lett., 22, 1619-1624.
No PubMed abstract available
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
1-cyclohexyl-3-(morpholinyl-4-ethyl)-carbodiimide 40 mM, 50% inhibition after 20 min, 100 mM, 44% inhibition after 10 min in the presence of 1% starch Thermomyces lanuginosus
Cu2+ 5 mM, 90% inhibition Thermomyces lanuginosus
Fe3+ 5 mM, 90% inhibition Thermomyces lanuginosus
N-bromosuccinimide 1 mM, complete inhibition after 5 min Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 starch 0.5 mg/ml, 50°C, pH 5.0 Thermomyces lanuginosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activation Thermomyces lanuginosus
Ca2+ activation Thermomyces lanuginosus
Cd2+ activation Thermomyces lanuginosus
Mg2+ activation Thermomyces lanuginosus
Mn2+ activation Thermomyces lanuginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 gel filtration Thermomyces lanuginosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Thermomyces lanuginosus potato starch maltose + maltotriose major products ?

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
 strain ATCC 34626
-

Purification (Commentary)

Purification (Comment) Organism
2-propanol, Mono Q, Superose 12 Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
349
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 55% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
 ?
amylose + H2O 73% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
 ?
beta-cyclodextrin + H2O 27% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
 ?
maltodextrin + H2O 100% of activity with potato starch Thermomyces lanuginosus maltooligosaccharides
-
 ?
maltoheptaose + H2O 43% of activity with potato starch Thermomyces lanuginosus maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose
-
 ?
maltohexaose + H2O 45% of activity with potato starch Thermomyces lanuginosus maltopentaose + maltotetraose + maltotriose + maltose
-
 ?
maltooligosaccharides + H2O 11% of activity with potato starch Thermomyces lanuginosus maltohexaose + maltopentaose + maltotetraose + maltotriose + maltose
-
 ?
maltopentaose + H2O 26% of activity with potato starch Thermomyces lanuginosus maltotetraose + maltotriose + maltose
-
 ?
maltotetraose + H2O 46% of activity with potato starch Thermomyces lanuginosus maltotriose + maltose
-
 ?
maltotriose + H2O 6% of activity with potato starch Thermomyces lanuginosus maltose + D-glucose
-
 ?
starch + H2O potato starch Thermomyces lanuginosus maltose + maltotriose major products ?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 32% and 94% loss of activity after 2 h and 18 h, respectively Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermomyces lanuginosus