Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 25-30% inhibition between pH 8.0 and 10.0 | Bacillus sp. (in: Bacteria) | |
Mn2+ | 1 mM, 20% inhibition | Bacillus sp. (in: Bacteria) | |
additional information | not inhibited by 100 mM EDTA | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Bacillus sp. (in: Bacteria) | soluble starch | maltotriose + maltohexaose + maltoheptaose + maltose | first two are major products, maltohexose and maltose are intermediate products | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
isolate KSM-K38 | - |
Oxidation Stability | Organism |
---|---|
strongly resistant to chemical oxidation, 20% loss of activity after 1 h incubation with 1.8 M H2O2 | Bacillus sp. (in: Bacteria) |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Toyopearl, Toyopearl HW-55F | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4221 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | from potato, 74% of activity with soluble starch | Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
amylose + H2O | 65% of activity with soluble starch | Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
dextrin + H2O | 27% of activity with soluble starch | Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
glycogen + H2O | from bovine muscle, 58% of activity with soluble starch | Bacillus sp. (in: Bacteria) | maltooligosaccharides | - |
? | |
starch + H2O | soluble starch | Bacillus sp. (in: Bacteria) | maltotriose + maltohexaose + maltoheptaose + maltose | first two are major products, maltohexose and maltose are intermediate products | ? | |
starch + H2O | soluble starch | Bacillus sp. (in: Bacteria) | maltotriose + maltohexaose + maltoheptaose + maltose | maltotriose and maltohexaose are major products, maltoheptaose and maltose are intermediate products | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
AmyK38 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
no loss of activity after 60 min | Bacillus sp. (in: Bacteria) |
50 | - |
80% loss of activity after 30 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | more than 50% of maximal activity between pH 6.5 and pH 10.0 | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | 20% loss of activity after 30 min at 40°C | Bacillus sp. (in: Bacteria) |