Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | creation of a chimeric enzyme Amy1A/3D, which has 158 amino acid residues of the N-terminus of isoenzyme Amy1A and 252 amino acid residues of the C-terminus of isoenzyme Amy3D | Oryza sativa |
N240Q | mutant of isoenzyme Amy1A | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
two major isoenzymes: Amy1A and Amy3D | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | isoenzyme Amy1A has an N-linked carbohydrate chain in the mature protein, isoenzyme Amy3D and chimeric enzyme Amy1A/3D do not contain N-linked carbohydrate chain | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + H2O | - |
Oryza sativa | additional information | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
isoenzyme Amy1A shows the highest thermostability, mutant enzyme N240Q of isoenzyme Amy1A shows almost identical thermostability to those of Amy3D and Amy1A/3D | Oryza sativa |