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Literature summary for 3.2.1.1 extracted from

  • Iefuji, H.; Chino, M.; Kato, M.; Iimura, Y.
    Raw-starch-digesting and thermostable alpha-amylase from the yeast Cryptococcus sp. S-2: purification, characterization, cloning and sequencing (1996), Biochem. J., 318, 989-996.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Cryptococcus sp. (in: Fungi)

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 51% inhibition Cryptococcus sp. (in: Fungi)
Cu2+ 1 mM, 52% inhibition Cryptococcus sp. (in: Fungi)
Hg2+ 1 mM, 25% inhibition Cryptococcus sp. (in: Fungi)
Zn2+ 1 mM, 87.6% inhibition Cryptococcus sp. (in: Fungi)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Cryptococcus sp. (in: Fungi)

Organism

Organism UniProt Comment Textmining
Cryptococcus sp. (in: Fungi)
-
-
-
Cryptococcus sp. (in: Fungi) S-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cryptococcus sp. (in: Fungi)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2539
-
-
 Cryptococcus sp. (in: Fungi)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + H2O short chain, DP 17 Cryptococcus sp. (in: Fungi) additional information
-
 ?
amylose + H2O short chain, DP 17 Cryptococcus sp. (in: Fungi) S-2 additional information
-
 ?
starch + H2O
-
 Cryptococcus sp. (in: Fungi) additional information main products of the 6 h reaction and the 18 h reaction are glucose, maltose, maltotriose and maltotetraose ?
starch + H2O
-
 Cryptococcus sp. (in: Fungi) S-2 additional information main products of the 6 h reaction and the 18 h reaction are glucose, maltose, maltotriose and maltotetraose ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Cryptococcus sp. (in: Fungi)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in absence of CaCl2 Cryptococcus sp. (in: Fungi)
60
-
-
 Cryptococcus sp. (in: Fungi)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 37°C, hydrolysis of soluble starch Cryptococcus sp. (in: Fungi)