Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Juge, N.; Svensson, B.; Williamson, G.
    Secretion, purification, and characterisation of barley alpha-amylase produced by heterologous gene expression in Aspergillus niger (1998), Appl. Microbiol. Biotechnol., 49, 385-392.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
secreted and correctly processed by Aspergillus niger Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 amylose DP 17, malt enzyme, calculated as low molecular weight products Hordeum vulgare
0.45
-
 amylose DP 17, recombinant enzyme, calculated as low molecular weight products Hordeum vulgare
1.16
-
 2-chloro-4-nitrophenyl beta-D-maltoheptaoside malt enzyme Hordeum vulgare
2.49
-
 2-chloro-4-nitrophenyl beta-D-maltoheptaoside recombinant enzyme Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl beta-D-maltoheptaoside + H2O
-
 Hordeum vulgare ?
-
 ?
amylose + H2O short chain, DP 17 Hordeum vulgare additional information
-
 ?
starch + H2O soluble starch Hordeum vulgare additional information
-
 ?
starch + H2O insoluble starch Hordeum vulgare additional information
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Hordeum vulgare
40.4
-
 2-chloro-4-nitrophenyl beta-D-maltoheptaoside malt enzyme Hordeum vulgare
63.1
-
 2-chloro-4-nitrophenyl beta-D-maltoheptaoside recombinant enzyme Hordeum vulgare
144
-
 amylose DP 17, malt enzyme, calculated as low molecular weight products Hordeum vulgare
185
-
 amylose DP 17, recombinant enzyme, calculated as low molecular weight products Hordeum vulgare